摘要
目的:探讨重楼总皂苷对细菌内毒素脂多糖(LPS)诱导大鼠腹腔巨噬细胞(PM)分泌TNF-α、IL-1β的影响。方法:分离并纯化大鼠腹腔巨噬细胞(PMΦ),与重楼总皂苷共同孵育,2小时后予LPS(100ng/ml)诱导PMΦ释放TNF-α、IL-1β,以ELISA酶联免疫吸附测定法检测上清液中TNF-α、IL-1β浓度。结果:重楼总皂苷对LPS诱导PMΦ释放TNF-α、IL-1β具有显著的抑制作用(P<0.01)。结论:重楼总皂苷可以抑制腹腔巨噬细胞分泌TNF-α、IL-1β的活性。
Objective： To study the effect of Rhizoma Paridis total saponins on TNF-α and IL-1β secretion in rat peritoneal macrophages induced by lipopolysaccharide. Methods： The rat peritoneal macrophages was isolated and purified, being cultivated with Rhizoma Paridis total saponins. 2 hours later, LPS （100mg/ml） was given to inereas the releasing of TNF-α and IL-1β induced by PMФ. The concentration of TNF-α and IL-1β in supematant was determined by ELISA. Results： The LPS induced secretion of TNF-α and IL-1β in PMФ could be significantly inhibited by Rhizoma Pardis total saponins （ P 〈 0.01 ）. Conclusion： The activity of TNF-α and IL-1β secreted bv PMФ could be inhibited bv Rhizoma Paridis ＇total saponins.
出处
《四川中医》
北大核心
2008年第3期14-16,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
贵州省科技厅
编号:黔科合丁字[2005]2075号
