摘要
为探索菌根技术在兰科植物保育中的应用,以野生五唇兰的内生菌根真菌和五唇兰组培苗为材料,进行组培瓶苗和盆栽苗的菌根化研究。利用固体培养的菌丝体与五唇兰无菌组培苗进行共生培养,15d后,根染色法检测结果表明,真菌能够成功侵染根,菌根化苗的移栽成活率均达到100%(对照为63.33%)。种植90d后各菌株处理苗的鲜样质量增长率均高于对照,其中F29菌株处理苗的鲜样质量增长率高于对照31.3%。在大棚栽培条件下,利用液体菌剂对盆栽苗进行菌根化培养。90d后各菌株处理苗的成活率和鲜样质量增长率均高于对照,其中F29菌株处理成活率比对照高出35%,鲜样质量增长率比对照高33.24%。
It aims at the application of mycorrhization technique in the orchid conservation. The endogenetic fungi from the root of the wild plant of Doritis pulcherrima Lindl. were inoculated with seedlings of D. pulcherrima in flask and in pot. Firstly, the symbiotic cultivation of aseptic seedlings with the myeelium of solid culture was carried out in flask. According to the method of root coloration, the result showed that the fungi can successfully infect the root of D. pulcherrirna seeding after 15 d, with 100% survival rate, while that of the control without mycorrhizal fungi was 63.33%. After 90 d, the fresh mass of these seedlings treated with fungi were all higher than the control, especially as for the treatment with fungi 1729, there were even 31.3% higher than the control. Secondly, seedlings were also cultivated with the liquid strain in pot. After 90 d, both the survival rate and the fresh biomass of the mycorrhization seedlings were higher than those of the control. For the mycorrhization seedlings with F29, they have 35% survival rate and 33.24% fresh mass higher than those of the control, respectively. The results suggested that there was remarkable or significant difference between the inoculated plants and the control.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第4期571-576,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30660153)
华南热带农业大学院校科技基金项目(Rndy0605
Rnd0612)
关键词
兰花
五唇兰
内生菌根真菌
育苗
orchid
Doritis pulcherrima Lindl.
endomycorrhizal fungi
seedling cultivation
