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嘧菌酯对番茄早疫病菌的抑制作用 被引量：31

Inhibitory Effect of Azoxystrobin on Alternaria solani
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摘要 采用菌丝生长速率法和孢子萌发法分别测定了嘧菌酯对番茄早疫病菌Alternariasolani菌丝生长的抑制作用及其对分生孢子萌发的影响，同时测定了其对菌丝呼吸速率的影响。结果表明，嘧菌酯抑制番茄早疫病菌分生孢子萌发的作用强于对菌丝生长的抑制作用。抑制菌丝生长的剂量．反应曲线与抑制菌丝呼吸的剂量．反应曲线趋势相似，但菌丝在经嘧菌酯处理5-7h后呼吸速率恢复到对照水平，相比之下，嘧菌酯却能一直抑制菌丝的生长。因此，采用孢子萌发法测定番茄早疫病菌对嘧菌酯的敏感性比用菌丝生长速率法测定更为适宜。水杨肟酸与嘧菌酯在抑制番茄早疫病菌菌丝生长和分生孢子萌发方面有协同作用，对菌丝生长的平均协同系数为7．24。随处理时间延长，药剂对菌丝耗氧的抑制作用下降，但仍抑制菌丝生长。研究表明：呼吸作用对药剂的敏感性随着处理的延长而下降的机理不是因为旁路氧化途径增强，也不是因为基质中药剂效力的下降，而是存在其它机制。 Inhibitory activity of azoxystrobin against mycelial growth and conidia germination of Alternaria solani was tested and the effect of azoxystrobin on respiration in mycelial growth ofA. solani was assessed by testing the oxygen consumption. The results showed that the inhibitory activity of azoxystrobin against conidial germination was much stronger than that against mycelial growth. The dose-response curves of azoxystrobin inhibiting mycelial growth and oxygen consumption of the fungus were parallel to each other. However, oxygen consumption restored to the normal level of control after 5 - 7 hours treatment with azoxystrobin, and the restored oxygen consumption was insensitive to azoxystrobin. By contrast, azoxystrobin could inhibit mycelial growth of A. solani all the times. Therefore,it is more appropriate to determine sensitivity of A. solani to azoxystrobin and monitor the resistance by conidial germination test than by mycelial growth. Salicylhydroxamic acid （SHAM） could strengthen the inhibitory effect of azoxystrobin against mycelial growth and conidia germination of A. solani and the mean synergistic ratio for mycelial growth was 7.24. As treatment time went on, the respiration inhibition on the mycelia decreased, while the mycelial growth was still inhibited strongly. The results showed that the sensitivity decrease of mycelial respiration to azoxystrobin was not caused by induction of alternative respiration or degradation of the fungicide in medium.
作者 张晓 张艳军 陈雨 周明国
机构地区 南京农业大学植物病理学系
出处 《农药学学报》 CAS CSCD 2008年第1期41-46,共6页 Chinese Journal of Pesticide Science
基金 国家重点基础研究发展规划("973"计划)项目(2006CB101907)
关键词 嘧菌酯 番茄早疫病菌 抑制作用 呼吸作用 azoxystrobin Alternaria solani inhibitory effect oxygen consumption
分类号 S482.2 [农业科学—农药学]
  • 相关文献

参考文献16

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二级参考文献17

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共引文献17

同被引文献381

引证文献31

二级引证文献164

农药学学报
2008年 第1期

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