摘要
用农杆菌介导方法,将甜菜坏死黄脉病毒外壳蛋白(BNYVV CP)基因和丛根病外壳蛋白(BBSVCP)基因转化甜菜不同品系茎节外植体。以卡那霉素作为筛选标记基因,获得抗卡那霉素再生植株。再生植株经PCR扩增,获得目的基因大小的DNA片段,初步证明为转基因植株。
BNYVV CP and BBSV CP gene were transformed into sugarbeet of diferent variety using agrobacterium tumfaciens - mediated method. From kanamycin resistance regenerate plant, DNA fregment were obtained by PCR detection, transgenic plant were preliminarily proved.
出处
《中国甜菜糖业》
2008年第1期12-17,共6页
China Beet & Sugar
基金
国家自然科学基金项目(30560080)
关键词
甜菜
遗传转化
PCR
surgarbeet
transformation
PCR
