摘要
目的：评价高效液相色谱定量检测谷物中赭曲霉毒素A的分析方法,为监测粮谷类食品中赭曲霉毒素A的污染水平提供技术支持。方法：利用液-液分离前处理技术,选用C18反相柱（250×4.6 mm）,以乙腈：水：乙酸=99：99：2为流动相,流速1 ml/min,荧光检测器（激发波长333 nm,发射波长460 nm）定量,柱温25℃。结果：以准确度和精密度评价该方法,其中变异系数均低于10%,回收率介于77.61%~95.66%之间。结论：与文献中所采用免疫亲和柱净化样品的方法比较,本法操作简便、灵敏度高、重现性好、结果可靠、经济,可用于谷物中赭曲霉毒素A定量检测。
Objective：To establish and evaluate a high-performance liquid chromatographic method for the analysis of ochratoxin A at low levels in samples of grains and provide technology for knowing the contamination level of OA in grain.Methods：The method involved a preconcentration step of liquid-liquid separation prior to chromatography.Chromatography conditions：C18 column（250×4.6 mm）,mobile phase was acetonitrile：water：acetic acid=99：99：2,the velocity of flow was 1 ml/min,fluorescence detector（exitation wavelength 333 nm,emission wavelength 460 nm）,the temperature of column was 25℃.Results：The method was evaluated for accuracy and precision with relative standard deviation lower than 10%,Recovery of Ochratoxin A added to grain is range 77.61%~95.66%.Conclusion：The HPLC method is operated simply,received high sensitivity and good reproducibility.Accordingly The method can be used to determined ochratoxin A in samples of grains.
出处
《中国卫生检验杂志》
CAS
2008年第1期12-13,39,共3页
Chinese Journal of Health Laboratory Technology
