摘要
目的观察碱性成纤维细胞生长因子（bFGF）对弥漫性脑损伤（DBI）组织细胞间黏附分子-1（ICAM-1）表达的影响，探讨bFGF的脑保护机制。方法按照Marmarou法建立大鼠DBI模型。干湿重法和荧光实时定量PCR分别测定脑外伤及bFGF预处理大鼠脑组织含水量和ICAM-1mRNA的表达。结果bFGF预处理组伤后不同时间点的脑组织含水量和ICAM-1mRNA表达趋势与外伤组类似。同一时间点预处理组含水量比外伤组下降，但高峰期延迟至48h出现。在6，12，24，48和72h时间点外伤组和预处理组含水量差异有统计学意义（P〈0．05）。在6，12，24，48，72h和7d时间点两组ICAM-1mRNA的表达差异有统计学意义（P〈0．05或P〈0．01）。结论bFGF预处理能下调外伤后ICAM-1mRNA的表达，这可能是其脑保护作用机制之一。
Objective To observe the effect of bFGF on expression of intercellular adhesion molecule-1 （ICAM-1） after diffuse brain injury （DBI） in rats and discuss its mechanism of cerebral protection. Methods The rat model of diffuse brain injury was established by Marmarou＇ s method. Drywet weight method and real-time quantitative PCR were used to detect the water content and expression of ICAM-1 mRNA in the brain tissue after DBI and pretreatment with bFGF respectively （ DBI group and pretreatment group）. Results The water content and ICAM-1 mRNA expression tendency in two groups at various time points were similar. Compared with DBI group, the water content was decreased at the same time point in pretreatment group, with a significant statistical difference at 6, 12, 24, 48 and 72 hours （ P 〈0. 05 ） and the peak was delayed to the 48th hour. There was a significant statistical difference in expression of ICAM-1 mRNA at 6, 12, 24, 48, 72 hours and 7 days between two groups （P 〈 0. 05 or 〈 0. 01 ）. Conclusions bFGF may down-regulate ICAM-1 mRNA expression after DBI, which serves as one of the cerebral protection mechanisms.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2008年第1期33-36,共4页
Chinese Journal of Trauma
关键词
脑损伤
成纤维细胞生长因子
碱性
胞间黏附分子-1
Brain injuries
Fibroblast growth factor, basic
Intercellular adhesion molecule-1
