摘要
提取菟丝子种子植物蛋白用高效毛细管电泳法进行生药鉴定。实验表明，同属不同种菟丝子的碱溶性或酸溶性蛋白在成分和含量上均存在显著差异。根据其种子植物蛋白的电泳谱图，可有效地鉴别菟丝子的来源。运用此法对１３个生药样品进行了鉴定。
A method for identification of semen Cuscutae , a traditional Chinese herb, was developed. The proteins in semen Cuscutae were extracted under acid or basic conditions and separated by high performance capillary electrophoresis. The electrophoretograms of acidic or basic extractants from Cuscuta chinensis Lam., Cuscuta australis R.Br. and Cuscuta japonica Choisy showed significant differences, which can be used to identify the three different semen Cuscutae . The results of the identification for 13 pharmacognosical samples agreed well with those of scanning electronic microscopy and tissue microanalysis.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第7期549-552,共4页
Acta Pharmaceutica Sinica
关键词
菟丝子
高效毛细管电泳法
生药鉴定
Semen Cuscutae
High performance capillary electrophoresis
Pharmacognosical identification
