摘要
目的:探讨苦荬菜组织培养和植株再生条件。方法:以苦荬菜叶片为外植体,应用正交设计方法,对愈伤组织的诱导和分化培养基进行优化筛选。结果:最佳的愈伤组织诱导培养基为MS+2,4-D 1.5 mg.L-1+6-BA1.5 mg.L-1+NAA1.0 mg.L-1+IBA1.5 mg.L-1+KT1.5 mg.L-1,最佳的不定芽诱导培养基为MS+2,4-D 0.2 mg.L-1+6-BA0.5 mg.L-1+NAA 0.5 mg.L-1+IBA 0.5 mg.L-1+KT0.5 mg.L-1,不定芽经附加IBA0.1 mg.L-1的1/4 MS培养基生根后移栽成活,获得再生植株。结论:建立了一套有效的苦荬菜组织培养再生体系。
Objective： To study the callus induction and plant regeneration of Ixeridium sonchifolium. Method： By using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized. Result： The optimal medium for callus induction was MS＋2,4-D 1.5mg·L^-1＋6-BA 1.5mg·L^-1＋NAA 1.0mg·L^-1＋IBA 1.5mg·L^-1＋KT 1.5mg·L^-1, the optimal medium for inducing adventitious bud was MS＋2,4-D 0.2mg·L^-1＋6-BA 0.5mg·L^-1＋NAA 0.5mg·L^-1＋IBA 0.5mg·L^-1＋KT 0.5mg·L^-1. Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg·L^-1. Conclusion： An efficient system for plant regeneration of I. sonchifolium was established.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第4期368-371,共4页
China Journal of Chinese Materia Medica
基金
吉林省科技厅项目(20050903-1)
关键词
苦荚菜
组织培养
植株再生
正交试验
Ixeridium sonchifolium
tissue culture
plant regeneration
orthogonal test
