摘要
背景与目的:探讨染色体着丝粒点(Cd)的变异与人绒癌细胞JEG-3和人卵巢畸胎瘤细胞PA-1染色体非整倍性畸变的关系。材料与方法:肿瘤细胞株传代后用常规方法制备染色体,用直接法制备胚胎绒毛细胞染色体标本,采用Cd-NOR同步银染分析技术研究JEG-3细胞和PA-1细胞染色体Cd的变异。结果:JEG-3细胞染色体Cd缺失率为1.43%、Cd迟滞复制率为0.21%、小Cd率为1.52%、Cd-NOR融合率为1.16%、与正常人胚胎绒毛细胞染色体Cd相比较,JEG-3细胞染色体Cd缺失、Cd-NOR融合率显著升高(P<0.0125),而与小Cd、Cd迟滞复制率间差异无统计学意义(P>0.0125)。PA-1细胞染色体Cd缺失率为1.22%、Cd迟滞复制率为0.95%、小Cd率为1.81%、Cd-NOR融合率为1.03%、与正常人胚胎绒毛细胞染色体Cd相比较,PA-1细胞染色体Cd缺失率、Cd迟滞复制率、Cd-NOR融合率均显著升高(P<0.0125),而与小Cd的差异无统计学意义(P>0.0125)。结论:JEG-3细胞染色体非整倍性畸变可能主要与Cd缺失、Cd-NOR融合有关;PA-1细胞染色体非整倍性畸变可能主要与Cd缺失、Cd迟滞复制和Cd-NOR融合有关。
BACKGROUND AND AIM： To study the relation between the centromeric dots（Cd）variation in JEG-3 cells and PA-1 cells and the chromosome aneuploidy aberration. MATERIALS AND METHODS： Chromosome of tumor cells were prepared with conventional method,chromosome of normal embryonic villi were treated directly. A Cd-NOR in-phase silver dye analysis technique modified by this laboratory was used to study centromeric dots（Cd） variation of JEG-3 cells and PA-1 cells. RESULTS： Frequency of Cd loss in JEG-3 cells was 1.43%, frequency of Cd duplication delay was 0.21%, frequency of small Cd was 1.52% and that of Cd-NOR fusion was 1.16%o Frequencies of Cd loss and Cd-NOR fusion in JEG-3 cells were significantly higher than those of normal embryonic villi（P〈O.O125） Frequency of Cd duplication delay and small Cd showed no difference between JEG-3 cells and normal embryonic villi cells. Frequency of Cd loss in PA-1 cells was 1.22%, of Cd duplication delay was 0.95%, of small Cd was 1.81%, and that of CA-NOR fusion was 1.03% o Frequencies of Cd loss, Cd duplication delay and Cd-NOR fusion in PA-1 cells were significantly higher than those of normal embryonic villi cells（P 〈0.0125）. Frequency of small Cd showed no difference between PA-1 cells and normal embryonic villi. CONCLUSION： Cd loss and Cd-NOR fusion might be related with aneuploidy aberration of JEG-3 cells. Cd loss, Cd duplication delay and Cd-NOR fusion might be related with aneuploidy aberration of PA-1 cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2008年第1期64-67,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
肿瘤细胞
着丝粒点
非整倍性畸变
tumor cells
centromeric dots
aneuploidy aberration
