摘要
鼻咽癌对我国南部居民的健康造成严重的威胁.为了研究鼻咽癌的发病机理,本研究采用了蛋白质组学技术分析和比较了鼻咽癌细胞系(HNE1和CNE1)与永生化的鼻咽上皮细胞系的蛋白质表达谱.采用双向凝胶电泳分离提取的全细胞蛋白质,通过PDQuest软件分析找出在肿瘤中表达变化的蛋白质点,用基质辅助激光解析电离飞行时间串联质谱(MALDI-TOF/TOF-MS)进行鉴定.共得到了15个在肿瘤细胞系中表达上调和18个在肿瘤细胞系中表达下调的蛋白质,并对其中一些蛋白质的表达进行免疫印迹的验证.这些表达差异的蛋白质与细胞的增殖和调亡、癌症的转移,细胞骨架,信号传导等有关.本研究鉴定了一批可能作为鼻咽癌治疗的药物靶标的蛋白质,并对研究鼻咽癌发病机理提供了相关的线索.
Nasopharyngeal carcinoma （NPC） poses serious health problems in Southern China and yet the molecular mechanism of the carcinogenesis remains unclear. We used modem proteomic technologies to compare the protein expression profiles between the NPC cell lines （HNE1 and CNE1 ） and an immortalized nasopharyngeal epithelial cell line NP69 to identify cancer related proteins. Cell lysates were separated by two- dimensional gel electrophoresis （2 DE） and analyzed by PDQuest software. The differentially expressed proteins were identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry （MALDI-TOF/TOF-MS）. We discovered 15 up-regulated proteins and 18 down-regulated proteins in both HNE1 and CNE1 cell lines compared with NP69. These proteins are correlative with various functions, such as cell proliferation, apoptosis, cancer metastasis, metabolism, cytoskeleton and signal transduction. Western blotting analyses were further carried out to verify the differential expression of individual proteins. Several identified proteins in our research might be used as potential molecular markers to understand the molecular mechanism of NPC development and metastasis, and might be used as candidate targets for NPC treatments.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2008年第1期11-19,共9页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家重点基础研究发展规划(973计划)资助(No2001CB5102)~~
关键词
鼻咽癌
细胞系
质谱
双向凝胶电泳
蛋白质组学
nasopharyngeal carcinoma
cell line
mass spectrometry
2 DE
proteomics
