摘要
目的:研究外消旋聚丙交酯(PDLLA)作为半月板组织工程支架材料应用的可行性。方法:采用胶原酶阶段消化法获取人胚半月板纤维软骨细胞,碱性成纤维细胞生长因子(bFGF)作用于第3代细胞,并用MTT比色法得出最佳效应浓度,扩增后的细胞种植于多聚赖氨酸修饰前、后的PDLLA上,在光镜、荧光显微镜、扫描电镜下观察PDLLA的亲水性、细胞吸附性和细胞增殖情况。结果:bFGF扩增人胚半月板细胞的最佳效应浓度为50μg/L(P<0.05);PDLLA亲水性及细胞吸附性欠佳,予多聚赖氨酸修饰后明显改善,细胞在PDLLA上生长良好,3周时已有大量细胞生长。结论:50μg/L的bFGF可用于体外大量扩增人胚半月板细胞;PDLLA经多聚赖氨酸修饰后可表现出良好的亲水性、细胞吸附性和体外生物相容性,可作为半月板组织工程细胞培养支架材料用。
Objective： To investigate the possibility of PDLLA as fibrocartilage cell scaffold in meniscus tissue engineering. Methods： Human fetal meniscal fibrochond-rocytes were digested with collagenase, the 3rd generation of cells were affected with basic fibroblast growth factor＇ （ bFGF）, and the dose-response was studied with MTT colorimetric method. The cells cultured onto PDLLA modified by poly-lysine or non-modified were observed with light micros-cope, fluorescence microscope, and scanning electronic microscopy. Results： Fibrocartilage cells affected by bFGF at the concentration of 50μg/L showed statistically higher proliferating rates than those in control group （P 〈0.05）. PDLLA showed good hydrophilia and cell adsorptivity after modified by poly-lysine. Big amount of fibrocartilage cells proliferated onto PDLLA in three weeks. Conclusions： bFGF can promote proliferation of human fetal meniscal cells. PDLLA has good hydrophilia, and adsorptivity, and is biologically compatible with fibrocartilage cells. So, it can be used as scaffold for meniscus tissue engineering.
出处
《贵阳医学院学报》
CAS
2007年第6期591-594,共4页
Journal of Guiyang Medical College
基金
贵州省卫生厅资助项目(G2005-16)
关键词
半月板
胫骨
软骨细胞
组织工程
胚胎
menisci, tibial
chondrocytes
tissue engineering
embryo
