摘要
D-氨基酸氧化酶(DAAO)是两步酶法催化头孢菌素C(CPC)生成7-氨基头孢霉烷酸(7-ACA)的重要酶之一。利用固定化DAAO催化CPC的转化液中,酮酸中间体随批次的增加逐渐减少,反应至第13批时,转化基本完全。当转化液pH由7.2升至7.6时,终止反应,转化率和收率分别达99.6%和93%。分次补加固定化DAAO,可连续转化132批。
D-Amino acid oxidase （DAAO） is one of the enzymes in the two-step bioconversion process of cephalosporin C （CPC） into 7-aminocephalosporanic acid （7-ACA）. By using the immobilized DAAO, the concentration of keto-CPC in reaction solution reduced with the increase of the reaction cycles, and keto-CPC was completely converted until the thirteenth cycle. Reaction was stopped when pH rose from 7.2 to 7.6, and the conversion rate and recovery were 99.6% and 93 %. Immobilized DAAO could be used for 132 cycles by immobilized DAAO fedbatch reaction.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2007年第12期846-848,共3页
Chinese Journal of Pharmaceuticals
基金
河北省科技厅重要成果(20070486)
