摘要
BACKGROUND： Quantitative pharmaco-electroencephalography can be used for studying the dose-effect and time-effect relationships of drugs affecting central nervous system. Therefore, it may become an effective means for monitoring the anesthetic degree of anesthetic drug in the operation. OBJECTIVE： To observe the dose-effect relationship of propofol influencing β2-band power of quantitative pharmaco-electroencephalography in rabbits. DESIGN： A randomized block design. SETTING： Department of Anesthesiology, Xuzhou Medical College. MATERIALS： Thirty-six healthy adult rabbits of either gender, weighing （2.4 ± 0.5 ） kg, of clean grade, were provided by the Laboratory Animal Center of Xuzhou Medical College. The involved rabbits were randomly divided into 3 groups with 12 in each by table of random digit： high-dose propofol group, moderate-dose propofol group, and low-dose propofol group. The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals. METHODS： This study was carried out in the Department of Anesthesiology, Xuzhou Medical College between August 1999 and April 2000. Rabbits in the high-dose propofol group, moderate-dose propofol group, and low-dose propofol group were injected with 10, 5 and 2.5 mg/kg propofol （ZENECA Company, British, Batch No. 032000）, respectively. Before and after intravenous administration of propofol, percentage of β2-band power of quantitative pharmaco-electroencephalography was measured, and the latent and persistent periods when rabbit righting reflex disappeared were observed by quantitative pharmaco-electroencephalography and power spectrum analysis. MAIN OUTCOME MEASURES： （1） Percentage of β2-band power of quantitative pharmaco-electroencephalography. （2）Latent period and persistent period of abolition of righting reflex of rabbits. RESULTS： Thirty-six rabbits were involved in the final analysis. （1）Effect of propofol on righting reflex of rabbits： Righting reflex disappeared within 1 minute after the rabbits being intravenously injected with propofol. The higher dose, the shorter latent period （r = - 0.94, P 〈 0.01）, and the longer persistent period （r =0.79, P 〈 0.01）. Both latent period and persistent period had good correlation with propofol dose. （2） Effect of propofol on the percentage of β2-band power of quantitative pharmaco-electroencephalography： In the low-dose propofol group, no significant changes in the percentage of β2-band power of quantitative pharmaco-electroencephalography existed between before and after administration within 30 minutes （P 〉 0.05）. In the moderate-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region except for left and right frontal areas was significantly decreased within 30 s to 5 minutes （P 〈 0.05）, and recovered to the level before administration 10 minutes later. In the high-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region was significantly decreased from 20 s to 5 minutes after administration （P 〈 0.05 - 0.01）, and that was gradually recovered to the level before administration 5 to 10 minutes after administration. This tendency was basically the same as the changes of latent period and persistent period. CONCLUSION： Propofol decreases the percentage of β2-band power of quantitative pharmacoelectroencephalography in dose-dependent manner, It indicates that β2-band power might become one of indexes for reflecting the anesthetic degree of propofol,
BACKGROUND： Quantitative pharmaco-electroencephalography can be used for studying the dose-effect and time-effect relationships of drugs affecting central nervous system. Therefore, it may become an effective means for monitoring the anesthetic degree of anesthetic drug in the operation. OBJECTIVE： To observe the dose-effect relationship of propofol influencing β2-band power of quantitative pharmaco-electroencephalography in rabbits. DESIGN： A randomized block design. SETTING： Department of Anesthesiology, Xuzhou Medical College. MATERIALS： Thirty-six healthy adult rabbits of either gender, weighing （2.4 ± 0.5 ） kg, of clean grade, were provided by the Laboratory Animal Center of Xuzhou Medical College. The involved rabbits were randomly divided into 3 groups with 12 in each by table of random digit： high-dose propofol group, moderate-dose propofol group, and low-dose propofol group. The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals. METHODS： This study was carried out in the Department of Anesthesiology, Xuzhou Medical College between August 1999 and April 2000. Rabbits in the high-dose propofol group, moderate-dose propofol group, and low-dose propofol group were injected with 10, 5 and 2.5 mg/kg propofol （ZENECA Company, British, Batch No. 032000）, respectively. Before and after intravenous administration of propofol, percentage of β2-band power of quantitative pharmaco-electroencephalography was measured, and the latent and persistent periods when rabbit righting reflex disappeared were observed by quantitative pharmaco-electroencephalography and power spectrum analysis. MAIN OUTCOME MEASURES： （1） Percentage of β2-band power of quantitative pharmaco-electroencephalography. （2）Latent period and persistent period of abolition of righting reflex of rabbits. RESULTS： Thirty-six rabbits were involved in the final analysis. （1）Effect of propofol on righting reflex of rabbits： Righting reflex disappeared within 1 minute after the rabbits being intravenously injected with propofol. The higher dose, the shorter latent period （r = - 0.94, P 〈 0.01）, and the longer persistent period （r =0.79, P 〈 0.01）. Both latent period and persistent period had good correlation with propofol dose. （2） Effect of propofol on the percentage of β2-band power of quantitative pharmaco-electroencephalography： In the low-dose propofol group, no significant changes in the percentage of β2-band power of quantitative pharmaco-electroencephalography existed between before and after administration within 30 minutes （P 〉 0.05）. In the moderate-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region except for left and right frontal areas was significantly decreased within 30 s to 5 minutes （P 〈 0.05）, and recovered to the level before administration 10 minutes later. In the high-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region was significantly decreased from 20 s to 5 minutes after administration （P 〈 0.05 - 0.01）, and that was gradually recovered to the level before administration 5 to 10 minutes after administration. This tendency was basically the same as the changes of latent period and persistent period. CONCLUSION： Propofol decreases the percentage of β2-band power of quantitative pharmacoelectroencephalography in dose-dependent manner, It indicates that β2-band power might become one of indexes for reflecting the anesthetic degree of propofol,
基金
the National Natural Science Foundation of China, No. 30471657,39970715
Social Development Plan Project of Science Committee of Jiangsu Province, No.BS2000052
the Natural Science Foundation of Jiangsu Province, No.BK2001143
