摘要
为了优化梭梭分化苗生根的培养基配方和培养条件,以剪去幼根的梭梭无菌苗茎、叶部分作为外植体,通过芽生芽途径获得了梭梭再生苗,重点对生根培养基和培养条件进行了系统的研究,根据试验结果得出结论:梭梭腋芽诱导培养基为MS+6-BA 2 mg.L-1,壮苗培养基为MS+6-BA 1.5 mg.L-1+GA3 1.0 mg.L-1,生根培养基为1/4MS+5%蔗糖+IBA0.2 mg.L-1+NAA 0.8 mg.L-1,生根率为74%。
In order to optimize the rooting medium composition and culture conditions, the aseptic seedling cut radicle of Haloxylon ammodendron was chosen for explants. Regenerated plant of Haloxylon ammodendron was obtained by manner of bud germination. Especially, the rooting medium and culture conditions were systematically researched. The bud induction medium of Haloxylon ammodendron was MS ＋6 -BA2mg · L^-1, strong sprout medium was MS ＋6 - BA1.5mg · L^-1 ＋ GA3 1.0mg L^-l and rooting medium wsa 1/4MS ＋ sucrose 5% ＋ IBA 0.2 mg · L^-1 ＋ NAA 0.8 mg · L^-1. Rooting percentage was 74%.
出处
《氨基酸和生物资源》
CAS
2007年第4期9-11,共3页
Amino Acids & Biotic Resources
基金
国家十五攻关项目"麻黄
柽柳和梭梭抗逆性植物材料的筛选与快速扩繁技术研究"
编号2005BA517A01
甘肃省科学事业费项目"利用植物组织培养技术筛选梭梭抗旱耐盐突变体"
编号QS031-C31-03
南京中医药大学合作-948创新项目"引进沙旱生植物良种应用技术创新与示范"
关键词
梭梭
组织培养
快速繁殖
Haloxylon ammodendron
tissue culture
rapid propagation
