摘要
目的为探讨癌细胞淋巴道转移的机制,观察小鼠肝癌移植瘤淋巴管Podoplanin免疫组化及酶组化表达。方法取(H22)肝癌细胞株,接种于小鼠腹股沟部皮下,第20 d后处死动物,取肿瘤及瘤周组织。应用5’-核苷酸酶(5’-Nase)和碱性磷酸酶(ALPase)双重染色及podoplanin免疫组化染色法,观察肿瘤及瘤周组织淋巴管的分布。结果动物模型在接种20 d时可见明显肿块;癌周边区可见较多棕色的毛细淋巴管和淋巴管。淋巴管腔较大且饱满,管壁薄,形态不规则。结论移植瘤周边区淋巴管增多,推测癌细胞淋巴道转移与癌周淋巴管的密度和扩张相关。
Objective Observe the expression of immunohistochemieal staining marker, podoplanin, and enzyme histochemical to explore the mechanism of lymphatic metastasis. Methods H22 cells were inoculated under the skin of groin of KM mice. Tumor tissue and periphery normal tissue were obtained after 20 days of inoculation. HE staining was used to observe the development of tumor. Podoplanin immunohistochemieal staining and 5＇ - Nase - ALPase double staining were performed at the same time to observe lymphatic vessel. Results The obvious mass can be seen under the groin of the mice in 20 days. In periphery region of carcinoma, the brown lymphatic capillaries and vessels can be found, with big cavity and, thin wall and irregular type. Conclusion The lymphatic vessel in periphery region of transplanted tumor increase, conjecture cancer cell lymphatic metastasis is correlated with lymphatic vessel density and expansion.
出处
《黑龙江医学》
2007年第12期911-913,共3页
Heilongjiang Medical Journal
基金
黑龙江省科技厅自然基金课题(D200625)
黑龙江省卫生厅科学技术课题(2006-471)
