摘要
目的:研究蛇足石杉原土壤微生物对其扦插生根的影响及其机制。方法:蛇足石杉分别扦插于原生境土壤和灭菌土壤上,调查其生根率,测定生根过程中吲哚乙酸(IAA)、酚类化合物(儿茶酚、槲皮素、绿原酸、阿魏酸)、多酚氧化酶(PPO)和过氧化物酶(POD)活性的变化。结果:原生境土壤微生物能够提高蛇足石杉的生根率,增加生根条数,提高生根过程中IAA、儿茶酚、绿原酸、阿魏酸的含量,影响了PPO,POD的活性。结论:原生境土壤上蛇足石杉扦插生根率高的重要原因是土壤微生物提高了蛇足石杉生根过程中IAA和儿茶酚、绿原酸、阿魏酸等酚类化合物含量。
Objective： To study the effects of soil microbes of habitats on the cuttings rooting of Huperzia serrata and its mechanism. Method： The cuttings of H, serrata were planted on the primary habitat soil and on the soil autoclaved, rooting rates were examined respectively. Changes of contents of phenolic compounds （catechin, quercetin, chlorogenic acid, ferulic acid） and indoleacetic acid （IAA）, and changes of activities of polyphenoloxidase （PPO） and peroxydase （POD） during the cuttings roofing were determined, Result： Soil microbes of primary habitat could increase the rooting rate and the number of roots, could increase contents of catechin, chlorogenic acid, ferulic acid and IAA during rooting, and could affect activities of PPO and POD. Conclusion： During the roofing higher contents of catechin, chlorogenic, ferulic acid and IAA caused by soil microbes of primary habitat are important factor for rooting rate.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第23期2478-2481,共4页
China Journal of Chinese Materia Medica
基金
中国科学院知识创新工程重大项目子课题(KSCX1-SW-11-6)
关键词
蛇足石杉
土壤微生物
扦插生根
Huperzia serrata
soil microbes
cuttigs rooting
