摘要
目的 探讨大肠癌中转录因子Prox-1的表达及对淋巴管生成、淋巴结转移的作用。方法 采用RT-PCR检测大肠癌正常组织、癌交界组织、癌组织中Prox-1的表达；用免疫组织化学sP法测定淋巴管密度（1ymphatic vascular density，LVD）。结果大肠癌组织、癌交界组织中Prox-1的表达水平均显著高于正常组织（P〈0．05）。癌交界组织中Prox-1的表达水平在DukesA＋B期和C＋D期、有无淋巴结转移之间比较差异有统计学意义（P〈0．05）。正常组织与癌交界组织的LVD比较差异有统计学意义（P〈0．05）；癌交界组织中LVD在DukesA＋B期和C＋D期、有无淋巴结转移之间比较差异有统计学意义（P〈0．05）。癌交界组织中Prox-1mRNA的表达水平与LVD呈正相关（r=0．54，P=0．018）。结论Prox-1的过度表达诱导淋巴管内皮细胞的分化、生成；Prox-1是预测肿瘤淋巴转移影响的有效因子之一，但不是独立因子；在进行预测时应选用癌交界组织。
Objective To study the expression of Prox-1 in colorectal carcinoma and its effects on lymphangiogenesis and lymphatic metastasis. Methods Twenty-five colorectal carcinoma patients admitted in our department from April to August 2004 were involved in this study. RT-PCR was used to detect the expression of Prox- 1 mRNA in normal tissues, paracarcinoma tissues and tumor tissues; immunohistochemical method （SP） was used to examine the lymphatic vessel density （LVD）. Results The expression of Prox-1 mRNA in tumor tissues and paracarcinoma tissues was significantly higher than that in normal tissues （ P 〈0.05 ）. The expression of Prox-I in paracarcinoma tissues in groups of Dukes A ＋ B, Dukes C ＋ D, and groups with or without lymph node metastasis showed significant difference （ P 〈 0.05 ）. There was statistical difference upon LVD between normal tissues and paracarcinoma tissues （ P 〈 0.05 ）. LVD of paracarcinoma tissues in groups of Dukes A ＋ B, Dukes C ＋ D, and groups with or without lymph node metastasis showed significant difference （ P 〈 0.05 ）. The expression of Prox-1 mRNA in paracarcinoma tissues had positive linear relation with LVD （ r = 0. 54, P = 0. 018 ）. Conclusions （1） The overexpression of Prox-1 induced the differentiation and growth of lymphatic endothelial cells. （2） Prox-1 is an effective factor in anticipating lyphatic metastasis, but not an independent factor. （3） Paracarcinoma tissue is preferable in anticipating lyphatic metastasis.
出处
《中华消化外科杂志》
CAS
CSCD
2007年第6期455-458,共4页
Chinese Journal of Digestive Surgery
