摘要
以组织细胞中提取的总ＲＮＡ为模板，加入ＴａｑＤＮＡ聚合酶和一对以ＴＧＦβ１ｃＤＮＡ为模板设计的引物，经变性、退火、延伸共３０次循环后，可以得到以ｃＤＮＡ为模板的扩增产物。实验证实ＴａｑＤＮＡ聚合酶具有逆转录酶活性，用一步常规聚合酶链反应即可获取逆转录聚合酶链反应的ＴＧＦβ１４６８ｂｐ扩增产物。
Total RNA extracted from the cell,together with Taq DNA polymerase and a pair of primer that was designed according to the sequence of the cDNA,was subjected to 30 cycles of amplification.The expected size of the product of the corresponding cDNA was obtained.The experiment demonstrated that Taq DNA polymerase has reverse transcriptase activity.A single step regular PCR could produce the amplified product of RT PCR under suitable condition.
出处
《中山医科大学学报》
CSCD
1997年第3期186-188,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
卫生部和广东省科委研究基金
关键词
聚合酶链反应
转化生长因子
β1
基因扩增
polymerase chain reaction/methods
DNA polymerases
transforming growth factor β 1
gene amplification
