摘要
目的:大肠杆菌O157:H7是近年来引发食物中毒事件的重要病原菌,对其建立灵敏、特异的多重PCR检测方法,是确保饮食安全,预防和控制大肠杆菌O157:H7传播的重要手段。方法:根据大肠杆菌O157:H7rfbE和fliC抗原基因的保守序列设计了2对引物,建立并优化了特异性检测大肠杆菌O157:H7的多重PCR体系,扩增产物分别为678 bp(rfbE)和560 bp(fliC)。结果:建立的多重PCR方法可以快速特异地检测出猪肉、牛肉等动物性食品中的大肠杆菌O157:H7污染,人工污染猪肉检测限达到2.2×102cfu/ml。结论:选择两对特异引物的多重PCR方法可简便、快速、特异地实现对动物性食品中大肠杆菌O157:H7的检测。
Objective： A multiplex PCR procedure was developed for specific detection of Escherichia coli O157 ： H7 in foods of animal origin to provide experimental basis for rapid diagnosis infection. Methods：Two pairs of primers were designed from its specific rfbE andfliC genes, Results： Fragments of 678 bp （rfbE） and 560 bp （fliC） could be amplified only from E, coli O157： H7,but not from other bacterial species by the PCR, The detection limit was 2.2×10^2 cfu/ml in artificially contaminated pork meat with incubation at 37℃ for 4 h, Condusion： This PCR procedure is rapid, specific and sensitive for detection of E. coli O157 ：H7 in food of animal origin such as pork and beef,
出处
《中国卫生检验杂志》
CAS
2007年第11期1951-1953,2068,共4页
Chinese Journal of Health Laboratory Technology
基金
广东省科技攻关项目(2004A20507004)
