摘要
研究了Alcalase水解大豆蛋白制备大豆蛋白寡肽时影响水解效果的各种因素,在此基础上通过正交试验确定了Alcalase水解大豆蛋白的水解条件:温度70℃,底物浓度60g/L,Alcalase酶与底物比为20μL/g,pH7.5,水解时间为4h。在此水解条件下,水解液水解度达到了24.1%。
The effects of several factors on the alcalase-catalyzed hydrolysis of soybean protein, such as the enzyme dosage, temperature, pH value and reaction time, were studied. The orthogonal test showed that the optimized hydrolysis conditions were as follow: pH value of 7.5, reaction temperature of 70℃, alcalase dosage of 20 μL/g protein, substrate concentration of 60 g/L, and total reaction time of 4 h. Under these conditions the hydrolysis degree of soybean protein reached 24.1%.
出处
《现代食品科技》
EI
CAS
2007年第11期51-53,共3页
Modern Food Science and Technology