摘要
目的:建立以反相高效液相色谱法测定黄芩苷脂质体的体外释放度的方法。方法:色谱柱为岛津C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(47∶53∶0.2),流速为1.0mL.min-1,检测波长为280nm,柱温为25℃,进样量为10μL。结果:黄芩苷检测浓度在1.01～80.80μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为100.05%,RSD=0.93%。结论:本方法灵敏、简便、准确,可用于黄芩苷脂质体的体外释放研究。
OBJECTIVE： To establish a HPLC method for the determination of in vitro drug release of Baicalin liposomes. METHODS： The separation was performed on a C18 column （150mm × 4.6mm, 5μm） at 25℃ ; the mobile phase was composed of methanol - water - phosohoric acid （47 ： 53 ： 0.2） at a flow rate of 1.0mL · min^-1.The ultraviolet wavelength was set at 280nm and the sample size was 10μL. RESULTS： The calibration curve showed good linearity in the range of 1.01 80.80μg · mL^-l（r-= 0.999 8） and the average recovery rate was 100.05% （RSD = 0.93%） .CONCLUSION： The method is sensitive, simple and accurate, and applicable for the study of in vitro drug release of Baicalin liposomes.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第33期2594-2596,共3页
China Pharmacy
