摘要
[目的]通过开展室内质控,以外部对照质控血清监测检测结果的可靠性和重复性。[方法]针对北京吉比爱ELISA法试剂的特点,自己配制弱阳性外部对照质控血清,每次实验均带外部对照质控血清检测,绘制质控图,并评价实验结果的可靠性。[结果]前20次外部对照质控血清的S/CO值建立质控框架图,从21次开始外部对照结果的S/CO值进入质控状态,曾出现位移现象,经分析找到原因后,位移现象消失,质控图分布正常,实验结果可被接受。[结论]保证HIV抗体初筛实验检测的可靠性,必须建立一系列质控体系,并持续开展室内质控,同时应注意检测中常出现的问题,并及时解决。
[Objective]To verify the reliability and reproducibility of testing results by using exterior QC control serum in process of internal quality control（IQC）.[Methods]According to the characteristics of the Chinese domestic anti-HCV EIA kits（GBI-Biotech Co.Ltd）,a kind of self-made exterior quality control serum was prepared,which was weakly positive.Each diagnostic test was carried on with the exterior quality control serum as the control.After tests,quality control diagrams were drew to evaluate the reliability of experimental results.[Results]The S/CO values of the advanced 20 exterior quality control sera were used to establish the framework diagram of quality control（QC）.Then,the S/CO values of the following tests were used for quality control statistic.Deviation was found and the cause was analyzed.After being corrected,the distribution of the quality control statistical graph was normal and acceptable.[Conclusions]To ensure the reliability of the initial HIV antibody screening test,a series of quality control systems have to be established,and internal quality control has to be developed continuously.Besides,It is important to carry on internal quality control and resolve the problems emerging in the procedure of testing.
出处
《现代预防医学》
CAS
北大核心
2007年第22期4354-4355,共2页
Modern Preventive Medicine
关键词
室内质控
外部对照质控血清
质控图
Internal quality control
Exterior QC control serum
Quality control diagram
