摘要
首次较系统地研究白芷斑枯病菌的生物学特性,结果表明：菌丝生长和产孢的温度范围为10~35℃,最适温度为25℃;最适pH5~7;病菌在PDA培养基上能够很好的生长和产孢;对碳源的利用以双糖最佳,对氮源的利用以无机氨态氮最好;光照对病菌的生长和产孢无显著影响。分生孢子在无菌水中的萌发率极低,加入糖分或寄主汁液可显著提高孢子的萌发率;分生孢子萌发的温度范围为10~35℃,最适温度为25~28℃;相对湿度93%以上孢子才能萌发,以RH100%＋水中孢子萌发率最高;pH5~7最有利于孢子萌发。在最适条件下,病菌孢子8 h开始萌发,24 h萌发率达到90%以上。光照对分生孢子的萌发无显著影响。分生孢子的致死温度为50℃（10 m in）。
The biological characteristics of the pathogen was studied systematically.The results showed that the temperature rage for mycelium growth and sporulation was from 10 to 35 ℃ and the optimum temperature was 25 ℃ The most suitable pH value was 5-7.The pathogen could grow and sporulate well on PDA.The optimum carbon sources was sucrose,and the most suitable nitrogen sources was ammonium nitrogen.The light had not remarkable effect on the mycelium growth and sprulation.Conidia were able to germinate in sterile deionized water but at a low percentage,sugar and host juice stimulated conidial germination of the pathogen.Conidia germinated at 10℃ to 35℃,with optimum temperature between 25℃and 28℃.The optimal relative humidity was above 93 %,and the maximum rate of spore germination in water.The optimum pH value for sporulation was 5-7.In suitable condition,germination of conidia started within 8 hours and the percentage of germination reached above 90 %.The light had not substantial effect on spore germination.The lethal temperature of conidia was 50℃（10min）.
出处
《西南农业学报》
CSCD
2007年第5期1007-1011,共5页
Southwest China Journal of Agricultural Sciences
基金
四川省教育厅重点项目(四川主要地道中药材病虫害研究)
关键词
白芷斑枯病
白芷壳针孢
菌丝生长和产孢
孢子萌发
Angelica leaf spot
Septoria dearnessii
mycelium growth and sporulation
spore germination
