摘要
Aim A reliable and rapid HPLC method was developed for quantitative determination of coniferyl femlate, an ester of ferulic acid, with multiple pharmacological activities in Angelica sinensis and Ligusticum chuanxiong, two commonly used Chinese medicines. Methods The determination was achieved by using a Zorbax ODS C18 analytical column （250 mm×4.6 mm ID, 5 μm） at isocratic elution of 1% aqueous acetic acid and acetonitrile （1：1） with diode-array detection （318 nm）. The calibration curve of coniferyl femlate showed good linearity （r^2 = 0.9995） within the test range. Results The developed method showed good precision with intra- and inter-day variations of 0.22% - 1.16% and 0.86% - 2.62% between the levels of 0.380 - 0.038 mg·mL^-1, respectively. The repeatability represented as RSD of coniferyl femlate was less than 2.7% for three levels （0.2 - 1.0 g of Angelica sinensis）, and the recovery was 105.3% with RSD of 3.2%. Conclusion The validated method was successfully applied to quantify coniferyl femlate in 12 samples of Danggui and Chuanxiong.
目的建立一种快速可靠测定当归、川芎中活性成份阿魏酸松柏酯的HPLC方法。方法色谱条件:ZorbaxODSC18色谱柱(250mm×4.6mmID,μm),流动相采用1%醋酸与乙腈1:1等度洗脱,检测波长318nm。结果建立的HPLC方法日内和日间精密度分别为0.22%–1.16%和0.86%–2.62%,阿魏酸松柏酯在0.380–0.038mg·mL–1范围内线形关系良好(r2=0.9995),加样回收率为105.3%,RSD为2.7%。结论该方法能够准确、快速定量测定当归、川芎中阿魏酸松柏酯的含量,有利于提高对当归、川芎的质量控制。
基金
Macao Science and Technology Development Fund(049/2005/A-R1)
Jiangsu Key Laboratory for TCM FormulaeResearch, Nanjing University of Traditional Chinese Medicine (No.022021014003).
