摘要
紫外线(UV)照射金龟子绿僵菌(Metarhizium anisopliae)MA4菌株后,从中分离筛选到高抗多菌灵(MBC)的突变菌株。对其中部分突变菌株的抗药稳定性分析,突变菌株MA4-1在无多菌灵培养基中继代培养10代后,仍然能在600μg/mL的多菌灵浓度下生长,而菌株MA4只能在0.5μg/mL多菌灵浓度下生长。结果表明:突变菌株MA4-1抗多菌灵浓度提高了1200倍,且抗药性稳定。方差分析结果表明:菌株MA4与突变菌株MA4-1,MA4-2,MA4-3的几丁质酶活力不存在显著差异。
Carbendazim-resistant mutants of Metarhizium anisopliae strain（MA4） were generated in the laboratory by ultra-violet （UV）. The resistance of the generated mutants to carbendazim was investigated. The mutants grew well by asexual reproduction in PDA and also grew at the concentration of 600 μg/mL of MBC even after 10 times of subculture, but the sensitive strain（MA4） only grew at the concentration of 0.5 μg/mL MBC. The results showed that the mutants increased its resistance to carbendazim by 1 200 times and was very stable in resistance. At the same time, the ANOVA demonstrated no significant difference in chitinase activity between the strain MA4 and the mutants MA4-1, MA4-2 and MA4-3.
出处
《热带作物学报》
CSCD
2007年第2期97-101,共5页
Chinese Journal of Tropical Crops
基金
国家科技部"科技基础性工作和社会公益研究专项"(编号:2004DIB3J073)
科技部"国家科技基础条件平台工作"(编号:2004DKA30560)等资助
关键词
金龟子绿僵菌
UV
多菌灵
几丁质酶
Metarhizium anisopliae ultra violet carbendazim chitinase
