摘要
按照ISO4387标准进行吸烟,通过自行研制的捕集装置,以2-苯叔丁基硝酮(PBN)的四氯化碳溶液捕集气相自由基.将捕集到气相自由基的溶液先用液氮冷冻,再定时解冻以抑制其衰减,并有效地去除氧气对气相自由基定量分析的干扰,获得理想的测定重复性.用电子顺磁共振波谱仪(EPR)检测气相自由基,以2,2,6,6-四甲基哌啶-1-氧(TEMPO)作为标准样品,以碳酸钙稀释的煤粉作为校正样品,定量计算气相自由基.测定方法有较好的重复性和较高的灵敏度,可以满足日常卷烟烟气气相自由基的定量分析的需要.
An improved method was developed for the determination of the gas-phase free radicals in mainstream cigarette smoke. A trap device for the gas-phase free radicals was developed, which could be easily attached to and removed from a commercial linear smoking machine. N-t-Butyl-α-phenylnitrone （PBN） was used to trap the gas-phase free radicals and the formed adduct was detected with EPR technique. The adduct was immediately frozen in liquid nitrogen for degassing and stabilization of the adduct. 2,2,6,6-Tetramethyl-piperidinyl-l-oxy（TEMPO） and strong pitch was used as calibration samples to quantitatively analyze the gas-phase free radicals. The sampling, conditioning and selection of cigarettes in the test were accordant with ISO 4387. It was proved that the established method was highly sensitive, reproducible, practicable and accurate for the detection of the gas-phase free radicals in routine tests.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第10期1846-1851,共6页
Chemical Journal of Chinese Universities
基金
国家烟草专卖局烟草科学研究与技术开发项目(批准号:0707-5)
国家烟草专卖局标准化研究项目(批准号:2002B011)资助.
关键词
主流卷烟烟气
气相自由基
定量分析
电子顺磁共振
Main-stream cigarette smoke
Gas-phase free radicals
Quantitative analysis
Electron Paramagnetic Resonance （EPR）
