摘要
目的研究比较肝细胞肝癌中保罗样激酶1(PLK1)及上皮钙黏蛋白(E-cadhefin)的表达与临床病理因素关系及对预测肝癌移植术后复发的价值。方法应用 RT-PCR 检测 PLK1 及E-cadherin 在转录水平的表达趋势;应用免疫组织化学检测两者蛋白水平的表达情况,并与临床病理因素及术后无瘤生存期、复发率进行比较分析。结果在转录水平方面,50对肝癌及癌旁冰冻标本中PLK1及 E-cadherin 的表达阳性率分别为90.0%与96.0%,PLK1 mRNA 癌高于癌旁,E-cadherin 则无趋势;50例肝癌石蜡标本中 PLK1 阳性表达及 E-cadherin 低表达的阳性率分别为60.0%与50.0%,PLK1阳性表达仅与术前血清 AFP 相关,E-cadherin 降低与临床病理因素均无相关性,PLK1阳性表达与 E-cadherin 低表达之间相关,Kaplan-Meier 分析结果示 PLK1、E-cadherin、肝癌大小、门静脉癌栓、Edmondson 分级、术前血清 AFP 等均与复发相关,Cox 回归分析示仅 PLK1 有统计学意义。结论PLK1阳性表达与 E-cadherin 低表达预示肝癌移植术后有较高的复发率,且 PLK1可作为复发的独立风险因素。
Objectives To study the expression of Polo-like kinase 1 （PLK1） and E-cadherin in the tissues of hepatocellular carcinoma, and to disscuss the relationship between them and clinical-pathological features, and to evaluate their prognostic value of hepatocellular carcinoma after liver transplantation. Methods mRNA and protein expression of PLK1, E-cadherin were detected by RT-PCR and immunohistochemistry method respectively, the correlations of them with clinical-pathological data, tumor free time, recurrence rate were compared and analyzed. Results The mRNA expression was observed in 90. 0% for PLK1 and 96. 0% for E-cadherin, and higher in cancerous＇ tissues than paracancerous＇ of all cases for PLK1 but no trend for E-cadherin. The positive and decreased expression rate for PLK1 and E- cadherin was observed in 60.0% and 50.0% respectively, the positive PLK1 expression was correlated with preoperative serum et-fetoprotein （AFP） only （X2 = 4. 433, P = 0. 035 ）, while E-cadherin expression was associated with none of the clinical-pathological features. There was a correlation between the positive PLK1 and decreased E-cadherinexpression＂ （X2 = 5. 333, P = 0. 021 ） . PLK1 （ P = 0. 006 ） , E-cadherin （ P = 0. 019） and larger tumor （ P = 0. 019 ） , portal vein tumor thrombi （ P = 0. 030 ） , Edmondson grading （ P = 0. 019）, preoperative serum AFP（ P = 0. 020） were all correlated with recurrence rate under Kaplan-Meier analysis, while only PLK1 （ RR = 3. 104, P = 0. 009 ） had significant difference under Cox regression analysis. Conclusions The positive PLK1 expression and the decreased E-cadherin expression indicate higher recurrence rate of HCC after liver transplantation, and PLK1 is a independent risk factor.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2007年第19期1354-1358,共5页
Chinese Journal of Surgery
基金
国家"973"重点基础研究项目资助(2003CB515501)
关键词
癌
肝细胞
复发
保罗样激酶1
上皮钙黏蛋白
Carcinoma, hepatocellular
Recurrence
Pole-like kinase 1
E-cadherin
