摘要
目的:研究柴胡总皂苷对大鼠酒精性肝病(ALD)的预防作用及其机制。方法:SD大鼠随机分为3组:酒精组、柴胡皂苷组、正常对照组,利用白酒灌胃辅以高脂饲料方法建立大鼠ALD模型,同时给予柴胡总皂苷保护,12周末处死大鼠,测定有关指标。结果:柴胡总皂苷明显升高肝组织活性、降低丙二醛(MDA)含量,降低血清转氨酶(ALT、AST)活性和甘油三酯(TG)含量,与模型组比较差异显著(P<0.01);并且能明显改善由酒精引起的肝组织脂肪变性和小叶灶性坏死,使肝小叶结构趋于正常。结论:柴胡总皂苷对ALD有明显防护作用,其机制可能与柴胡总皂苷清除自由基抑制脂质过氧化反应有关。
Objective： To study the protective effect of saikosaponins （SS） on alcoholic liver disease （ALD） in rats and its mechanism, Methods ： SD rats were randomly divided into 3 groups： normal group, ethanol - fed group, SS interventional group. 56% distilled alcohol and high fat diet were griven to the rats of the ethanol - fed group and the SS group by stomach every day, the rats of SS group were administered with SS 0.2g ·kg-^1 once daily. Rats were saerificd after 12^th weeked. The level of serum alanine transaminase（ ALT）,partate aminotransferase （AST） and triglyceride （TG） were examined, The concentrations of malandialdehyde（MDA） and gtutathione peroxidase（ GSH -px） were estimated in liver tissue,The pahtological changes of liver tissue were examined by H. E.Results ： To compared with model group, SS cound heighten actives of GSH - px and reduce the content of MDA in liver tissue. Tl^e level of serum ALT,AST and content of TG were obviously decreased（ P 〈 0.01 ）, Hepatic injury was reversed. Conclusions： SS has beneficial protective effect on ALD in rats, the mechanisms may be contributed to eliminaing free radicals and reducing lipid peroxidation.
出处
《辽宁中医杂志》
CAS
北大核心
2007年第10期1492-1493,共2页
Liaoning Journal of Traditional Chinese Medicine
基金
河北省科技厅资助课题(05276101D-4)
