摘要
以180只3个品系的温岭草鸡为材料,采用PCR-RFLP方法测定了IGF-Ⅰ基因的3个SNPs座位,同时分析了它们对屠体性状的遗传效应。结果显示:PstⅠ、HinfⅠ和TaqⅠ识别座位分别发生T→C、C→A和C→T突变,每个SNPs座位各出现了3种基因型,其中A系在3个座位上均处于Hardy-Weinberg平衡状态(Ρ>0.05)。方差分析显示每个座位基因型对部分屠体性状都有极显著或显著的差异(Ρ≤0.01或0.01<Ρ≤0.05)。多重比较显示:在其中6个屠体性状上,每个座位3种基因型的最小二乘均值之间均有突变型>杂合型>野生型的关系,联合基因型QF/QF和QE/QF的最小二乘均值在4个屠体性状上显著高于(0.01<Ρ≤0.05)联合基因型PE/QE,每个座位突变等位基因都对部分屠体性状具有增效作用。A系和B系之间的遗传距离为0.005 3,聚类分析表明两系同为1枝。
Three SNPs loci in IGF- Ⅰ gene in 180 Wenling Grass-chicken including 3 lines were detected by PCR-RFLP method,and its genetic effects on carcass traits were also analyzed. The mutations of T→C,C→A and C→T were identified by Pst Ⅰ , Hinf Ⅰ and Taq Ⅰ , respectively. Three genotypes were acquired in each locus,and line A was at the Hardy-Weinberg equilibrium （P〉0.05） in each locus. The analysis of variance showed that the genotypes of each locus had significant or suggestive difference （P≤0.01 or 0. 01〈P≤0. 05） in partial carcass traits. The multiple comparisons indicated the Least Squares Means of 3 genotypes in each locus had the property of mutant genotype〉heterozygous genotype〉wild genotype in 6 carcass traits,and the com-genotypes QF/QF and QE/QF were suggestive higher （0.01〈P≤0.05） than PE/QE in 4 carcass traits. And the mutant allele of each locus had additive effect on partial carcass traits. The genetic distance between line A and B was 0. 005 3,indicating they were in the same branch through cluster analysis.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第10期1021-1026,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
浙江省温龄市科技局项目(I20503)
