摘要
【目的】选育具有抗缺铁能力的苹果属植物新种质。【方法】通过半定量式RT-PCR检测、印迹杂交、营养液pH的变化和低铁(铁浓度为10-6mol·L-1)水培条件下,根系和叶片的金属元素含量分析。【结果】在通过Southern杂交获得的9个转番茄铁载体蛋白基因八棱海棠(Malus robusta Rehd.)株系中,有6个转基因株系成功转录,目的基因在叶片和根系中都有表达。其中2个在缺铁情况下显著提高了根系总铁的含量和叶片中活性铁的含量,同时也大幅度提高了根系和叶片中锌的含量。【结论】转番茄铁载体蛋白基因八棱海棠株系表现出较强的抗缺铁胁迫能力。
【Objective】The study looked at breeding the new germplasm to iron-deficiency in Malus Mill.【Method】Scientists used the expression identify with half a fix to quantify RT-PCR and tissue printing mRNA Northern hybridization.The hydroponic experiment determined Fe,Mn,Zn content of leaves and roots of the transgenic lines and non-transgenic plants.【Result】Nine transgenic plants of Malus robusta were confirmed by Southern blot analysis.Six of them were successful transcript.The tolerance to iron-deficiency was only found in the two transgenic lines.In the transgenic lines,the total Fe content of the roots and the activated Fe content of the leaves were notably increased.The Zn content of the leaves and roots were also notably increased.Strong hybridization signals were observed in the leaves and roots of the transgenic Malus robusta which had been exposed to iron stress.【Conclusion】The tolerance to iron-deficiency was found in the two transgenic lines.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第9期2015-2020,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30370987)
江苏省应用基础资助项目(BJ200018)
关键词
八棱海棠
LeIRT2
缺铁胁迫
Malus robusta
LeIRT2
Tolerance to iron-deficiency
