摘要
目的探讨表皮生长因子受体(EGFR)与恶性上皮性卵巢肿瘤血管生成的关系。方法采用免疫组化PV-6000二步法,检测9例正常卵巢、10例良性上皮性卵巢肿瘤、9例交界性上皮性卵巢肿瘤和39例恶性上皮性卵巢肿瘤组织中EGFR蛋白的表达及微血管密度(MVD)。结果(1)交界性和恶性上皮性卵巢肿瘤中EGFR的阳性表达率及MVD计数均显著高于正常卵巢和良性上皮性卵巢肿瘤(P<0.01);(2)EGFR与恶性上皮性卵巢肿瘤的临床分期有关,Ⅲ～Ⅳ期的阳性表达率高于Ⅰ～Ⅱ期(P<0.05),与组织学分型、细胞学分级、患者年龄、腹水及淋巴结转移有无等临床病理参数无关;MVD计数与上述肿瘤临床病理参数均无关;(3)恶性上皮性卵巢肿瘤中EG-FR蛋白表达与MVD计数之间存在高度相关(r=0.685,P<0.01)。结论EGFR与恶性上皮性卵巢肿瘤的血管生成存在相关性,阳性表达强的组织微血管密集;EGFR可能参与了卵巢肿瘤的发生、发展过程,可作为卵巢恶性肿瘤的生物学标志物之一。
Objective To clarify the clinical relationship between EGFR expression and angiogenesis in ovarian epithelial carcinoma. Methods The EGFR protein expression and MVD by immunohistochemistry PV-6000 technique in 67 ovarian specimens were detected, including 9 norreal,10 benign,9 borderline and 39 malignant samples. Results （1） EGFR positive rate and MVD in the borderline and malignant ovarian specimens were higher than that in the normal and benign ones（P〈0.01）. （2）There was correlation between EGFR positive expression and clinical stage, for example, stage Ⅲ～Ⅳ had higher positive expression rate than stage Ⅰ～Ⅱ（P〈0.05） ;MVD had no correlation with the clinico-pathologic parameters. （3）There was a significant correlation between EGFR positive expression and MVD（r=0. 685,P〈0.01）. Conclusion EGFR may participate in the angiogenesis and development of ovarian epithelial carcinoma,which could be a usefully biological marker for ovarian neoplasms.
出处
《江西医学院学报》
2007年第4期8-11,15,共5页
Acta Academiae Medicinae Jiangxi
