摘要
目的观察人参皂苷Rb1和Re对乌头碱导致的心肌细胞损伤的影响。方法选用乳鼠心肌细胞,观察人参皂苷Rb1和Re分别与乌头碱配伍使用后,乳鼠心肌细胞上清心肌酶谱的改变,采用RT-PCR的方法,观察人参皂苷Rb1和Re与乌头碱配伍对心肌细胞钙离子通道基因Cav1.2 mRNA的影响。结果人参皂苷Rb1和Re可以有效对抗由乌头碱导致的心肌酶谱改变。同时,可以对抗乌头碱所致的钙离子通道基因Cav1.2 mRNA表达上调。结论人参皂苷Rb1和Re与乌头碱配伍具有减毒增效的作用,这可能与人参皂苷减轻了乌头碱所致的心肌细胞损伤和改善钙离子通道异常表达有关。
Abstract： Objective To investigate the protective effect of ginsenoside Rb1 and Re on injury of the neonate rat cardiomyocyte induced by aconitine alkaloids.Methods The influence of aconitine and ginsenoside Rb1, Re together in neonate rat cardiomyocyte was observed respectively. The influence of these components in neonate rat cardiomyocyte was examined by myocard zymogram. The expression of Ca^2＋ channel gene Cav1.2 mRNA of neonate rat cardiomyocyte aider treatment was observed by RT-PCR. The ginsenoside Rh1 and Re were able to decrease the release of AST and LDH, reverse Cav1.2 mRNA abundance induced by aconitine. Conclusion The ginsenoside Rb1 and Re which together with aconitine can alleviate the side effects and boost up the therapeutic effects of aconitine. The action mechanism may be relation with that the ginsenoside Rb1 and Re can decrease injuries of the neonate rat cardiomyocytes and abundant expression of Cav1.2 mRNA induced by aconitine.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第9期33-35,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家科技攻关计划(2004BA721A11)
