摘要
The enhanced biological phosphorus removal （EBPR） method is widely adopted for phosphorus removal from wastewater, yet little is known about its microbiological and molecular mechanisms. Therefore, it is difficult to predict and control the deterioration of the EBPR process in a large-scale municipal sewage treatment plant. This study used a novel strain isolated in the laboratory, Pseudomonas putida GM6, which had a high phosphate accumulating ability and could recover rapidly from the deteriorated system and enhance the capability of phosphorus removal in activated sludge. Strain GM6 marked with gfp gene, which was called GMTR, was delivered into a bench-scale sequencing batch reactor （SBR） of low efficiency, to investigate the colonization of GMTR and removal of phosphorus. After 21 days, the proportion of GMTR in the total bacteria of the sludge reached 9.2%, whereas the phosphorus removal rate was 96%, with an effluent concentration of about 0.2 mg L^-1. In the reactor with the addition of GMTR, phosphorus was removed quickly, in 1 h under anaerobic conditions, and in 2 h under aerobic conditions. These evidences were characteristic of EBPR processes. Field testing was conducted at a hospital sewage treatment facility with low phosphorus removal capability. Twentyone days after Pseudomonas putida GM6 was added, effluent phosphorus concentration remained around 0.3 mg L^-1, corresponding to a removal rate of 96.8%. It was therefore demonstrated that Pseudomonas putida GM6 could be used for a quick startup and enhancement of wastewater biological phosphorus removal, which provided a scientific basis for potential large-scale engineering application.
提高的生物的磷移动(EBPR ) 方法广泛地从废水为磷移动被采用，然而，很少对它的微生物、分子的机制被知道。因此，在一家大规模市政的污水处理工厂预言并且控制 EBPR 过程的恶化是困难的。使用的这研究在实验室孤立的新奇紧张， Pseudomonas putida GM6，它有高磷酸盐积累能力并且能从败坏的系统很快恢复并且在活性污泥提高磷移动的能力。与 gfp 基因标记的紧张 GM6，被称为 GMTR，被交付进定序低效率的批反应堆(SBR ) 的凳子规模，到调查 GMTR 的殖民和磷的移动。在 21 天以后，而磷移动率是 96% ，在污泥的全部的细菌的 GMTR 的比例到达了 9.2% ，与大约 0.2 mg L 的自河集中 ? 1。在有 GMTR 的增加的反应堆，磷快速被移开，在在厌氧的条件下面的 1 h，并且在在氧气的条件下面的 2 h。这些证据 EBPR 过程是特征的。现场测试与低磷移动能力在一台医院污水处理设备被进行。二十 -- 一天在 Pseudomonas putida GM6 被增加以后，自河磷集中在 0.3 mg L 附近留下了 ? 1，相应于 96.8% 的移动率。它因此被表明为废水的快开始和改进的那 Pseudomonas putida GM6couldbeused 生物的磷移动，它为潜在的大规模工程申请提供了一个科学基础。
基金
Project supported by the National Natural Science Foundation of China (Nos.30500010 and 50308011).
