摘要
从中国北方地区拓展杨树品种资源考虑,以哲引3号杨为母本,小青杨和北京杨为父本,对授粉后24～96 h的雌花序施加0.3%～0.5%的秋水仙碱溶液处理,获得了26株青杨杂种三倍体。其中尤以授粉后54～66 h时施加秋水仙碱处理的三倍体诱导效果最好,三倍体得率最高,达66.7%。与二倍体后代群体相比,青杨杂种三倍体具有一定的生长优势,其中哲引3号杨×北京杨三倍体2年生实生苗的地径和苗高平均值分别超出二倍体20%和5%;最优单株的地径和苗高分别达4.42 cm和2.77 m,超出二倍体平均值144%和43%。青杨派树种的三倍体诱导实践进一步证明,雌花序授粉后施加秋水仙碱处理是一种高效的三倍体诱导方法。
In order to enrich the resources of Populus varieties in north China,female catkins were treated with 0.3%-0.5% colchicine solution during 24-96 hrs after pollination in cross combinations including（P.pseudo-simonii × P.nigra var.lica） ×（P.× beijingensis） and（P.pseudo-simonii × P.nigra var. lica） × P.pseudo-simonii,and 26 triploid plants were obtained.The results showed that 54-66 hrs after pollination was the optimal period to induce triploids in these cross combinations,and the highest ratio of obtaining triploids was 66.7%.Compared with the diploid progeny,the growth of these allo-triploids showed certain superiority.Especially in（P.pseudo-simonii × P.nigra var.lica） ×（P.× beijingensis）,on average,the basal diameter and stem height of two-year-old triploid seedlings exceeded the ones of diploids by 20% and 5% respectively;the two indices of the best triploid in this cross combination were 4.42 cm and 2.77 m,which were 144% and 43% more than those of the average growth of diploids respectively.Induction of allo-triploids in Section Tacamahaca further proves that treating pollinated female catkins with colchicine is an efficient method to obtain triploid plants.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2007年第5期18-21,共4页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(30671708)
关键词
哲引3号杨
青杨派
授粉
秋水仙碱
三倍体
Populus pseudo-simonii × P.nigra var.lica,Section Tacamahaca,pollination,colchicine,triploid
