摘要
目的研究持续给予人甲状旁腺激素（human parathyroid hormone,hPTH）1-34对体外培养成骨细胞增殖与分化的干预作用。方法体外培养乳鼠成骨细胞,将细胞分为空白培养液对照组和10^-6、10^-7、10^-8mol·L^-1hPTH（1-34）3个不同剂量的给药组。每48h换1次液,换液时采用MTT法进行细胞增殖测定,同时检测细胞裂解液中的碱性磷酸酶（alkaline phosphatase,ALP）和骨钙素（bone gla protein,BGP）的水平,持续培养6d。结果持续给予hPTH1-34作用48h后,各给药组的MTT均明显高于对照组且各给药组间差异均有显著性（P〈0.05）,至第6天时各给药组则均明显低于对照组（P〈0.05）。ALP和BGP测定结果显示持续给予hPTH1-34作用48h后,10^-6mol·L^-1PTH给药组的BGP含量明显高于对照组（P〈0.01）,其余各组ALP及BGP含量与对照组比较差异虽无显著性,但均值高于对照组,至第6天各给药组均明显低于对照组（P〈0.05）。结论持续给予hPTH（1-34）对体外培养成骨细胞增殖与分化的影响与作用的时间和给药浓度密切相关。短期持续用药有促进作用,并且在10^-6-10^-8mol·L^-1浓度范围内呈剂量依赖性;长期持续用药抑制成骨细胞的增殖与分化,给药浓度越高抑制作用越强。
Objective To observe the effects of continuous stimulation with different doses of human parathyroid hormone 1-34 fragment （hPTH 1-34）on osteoblast. Methods The osteoblast from sucking rat was cultured and divided into PTH group and control group. Doses of 10^-6、10^-7、10^-8mol·L^-1 hPTH1-34 were supplemented respectively. Cell proliferation was measured by MTT method. Alkaline phosphatase （ALP） and bone gla protein （BGP） concentration inside cells were measured by chemical and radioimmunoassay methods. Results After 48 hours treated, the level of MTT was higher in PTH groups than that in control group （ P 〈 0.05 ）, and there was significantly difference among PTH groups （ P 〈 0.05） ; on the 6th day, the level of MTT was lower in PTH groups （P 〈 0.05 vs. control）. After 48 hours treated, the BGP level in 10^-6 mol·L^-1 PTH group was significantly higher than that in control group （ P 〈 0.01 ） ; on the 6th day, the level of ALP and BGP were lower in PTH groups （P 〈 0.05 vs. control）. Conclusion Continuous stimulation with hPTH 1-34 could improve the proliferation and differentiation of osteoblast in vitro pronouncedly in a short period, but inhibit when it stimulates the osteoblast for a long period. The effect depends on the doses of hPTH1-34 in a certain range.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第8期553-555,共3页
Chinese Journal of Osteoporosis
基金
天津市面上项目课题(05YFJMJC07600)
关键词
甲状旁腺激素
成骨细胞
碱性磷酸酶
骨钙素
Parathyroid hormone
Osteoblast
Alkaline phosphatase
Bone gla protein
