摘要
以壳聚糖为载体、戊二醛为交联剂对链霉菌LD048在液体培养的条件下合成的硫化物氧化酶进行了固定化,确定的最适固定化条件为:酶与载体的质量比为1∶4,交联剂质量分数0.05%,吸附3 h,交联6 h。固定化后酶的活性回收率为58.8%,每克固定化酶的催化活性达到2 000 U,Km值为2.43×10-5mol/L,固定化酶在100℃保温2 h,酶活仅下降6%。在以2.5 mg/(L.h)的容积负荷对固定化酶进行的装置化运行实验中,连续15天的催化操作后,固定化酶对S2-的去除效果仍在85%以上。
Sulfide-oxidizing enzyme synthesized by Streptomyces sp. D048 cultivated in liquid medium was immobilized on carriers of chitosan by covalent coupling with glutaraldehyde as cross-linker. Conditions of immobilization were optimized as： 1： 4 of the quality ratio of enzyme and carries,0.05% of the cross-linker concentration, 3 hours of absorbing and 6 hours of coupling.The catalytic activity recovery of this enzyme was 58.8% after immobilized,and the activity for per gram immobilized enzyme is up to 2 000 U.Km value of immobilized enzyme is 2.43 × 10^-5mol/L, and only 6% of activity decrease can be detected when immobilized enzyme was kept at 100℃ for 2 hours. Catalytic operation showed that the removing rate of immobilized enzyme to S^2- supplied by this test still maintained above 85% after 15 days of continuous catalytic operation in installed experiment for immobilized enzyme with 2.5 mg/（L·h） of volumetric load.
出处
《现代化工》
EI
CAS
CSCD
北大核心
2007年第8期45-47,49,共4页
Modern Chemical Industry
基金
山东省山东大学明星计划(济青科合字(2005)第106号)
