摘要
[目的]为了研究磁场处理对种子的影响,并为磁场处理技术在蔬菜生产中的应用及其生物机制的研究提供理论依据。[方法]将蚕豆种子浸泡24 h后进行磁场处理,磁感应强度分别为2004、006、008、00和1 000 mT,处理时间为5 min和10 min,随后测定蚕豆种子活力和幼苗体内过氧化氢酶、过氧化物酶活性。[结果]各磁场强度处理中,种子发芽势、发芽率、发芽指数和活力指数都高于对照,根、茎、叶中CAT、POD活性都比对照强;不同组织部位CAT、POD的活性不同,根的活性高于茎和叶,茎的活性最低。5个磁场强度中,又以600mT、800 mT为最佳。[结论]磁场对细胞膜具有一定的修复作用。磁场处理能提高酶的活性,可能与POD、CAT酶含有金属离子有关。金属离子在磁场的作用下发生定向排列,引起了酶构像的变化,进而激活了酶。
[ Objective] The purpose was to study the effect of magnetic treatment on seeds and supply the theoretical basis for the application of magnetic treatment technique in vegetable production and research on its biologic mechanism. [ Method] The broad bean seeds soaked for 24 h were treated by magnetic treatment with magnetic induction inteusian of 200, 400, 600, 8（D and 1 000 mT for5 min and 10 min, resp., and then the seed vigor of broad bean and activities of catalase （CAT） and peroxidase （POD） in young seedlings were determined. [ Result ] In each magnetic strength treatment, the seed germinating potential, germination rates, genninatian index and vitality index were all higher than that of CK and the activities of CAT and POD in root, stern and leaf were all stronger than that of CK. The activities of CAT and POD in different tissues were different. It was higher in root than that in stem and leaf, and the lowest in stem. In the 5 magnetic strength treatments, 600 mT and 800 mT were the best. [ Conclusion] Magnetic field had some remediation function on cell membrane and the enzyme activity enhancement in magnetic treatment might be related to POD and CAT, which contained metal ions. The magnetic effect made metal-ion produce directional arrangement, which caused conformational change of enzyme and then activated enzyme.
出处
《安徽农业科学》
CAS
北大核心
2007年第22期6723-6724,共2页
Journal of Anhui Agricultural Sciences
