摘要
对分离得到的D53、D56菌株经PCR扩增后,测定其16S rRNA基因序列,与基因库中基因序列进行同源性比较可知,D53、D56菌株与枯草芽孢杆菌的相似性达99%。结合其形态观察及生理生化实验综合分析,得出2株菌为枯草芽孢杆菌,并在Genebank中申请登陆号分别为DQ923482和DQ923483。对产淀粉酶透明圈的测定结果表明,D53、D56的产酶能力达到6.5 U/mL和8.2U/mL。
The sequence 16S rRNA gene of two isolated D53 and D56 strains was determined after PCR amplification. Homology comparison with gene sequence in gene bank proved that D53, D56 and Bacillus subtillis was 99% similarity. The two strains were identified to be Bacillus subtillis with morphological and physic-chemical analysis. Two ID numbers were applied for DQ923482 to D53 and DQ923483 to D56 in gene bank. The test of transparent circle by production of amylase proved that amylase-producing activity was 6.5 U/ml and 8.2 U/ml secreted by D53 and D56.
出处
《中国酿造》
CAS
北大核心
2007年第8期26-28,共3页
China Brewing
