摘要
目的研究抗SmD1抗体及其他特异性抗体在系统性红斑狼疮(SLE)诊断中的意义。方法测定了44例SLE及136例非SLE(包括干燥综合征、未分化结缔组织病、强直性脊柱炎、类风湿关节炎)患者血清中的自身抗体。利用免疫印迹法测定抗SmD1抗体、抗核小体抗体(ANuA)和抗SSA60 000抗体,间接免疫荧光法测定抗核抗体(ANA)和抗双链DNA(dsDNA)抗体,免疫斑点法检测抗Sm抗体。结果抗SmD1抗体在SLE患者中的阳性率为47.7%,高于抗Sm抗体阳性率18.2%,差异有统计学意义(P<0.05),而且其抗SmD1抗体的特异性达97.1%。结论抗SmD1抗体在狼疮的诊断中有很高的特异性,敏感性也高于抗Sm抗体。5种特异性抗体在SLE诊断中有明显的互补作用,联合检测可明显提高其对SLE诊断的敏感性。
Objective To investigate the significance of anti-SmD1 antibody and other auto-antibodies in systemic lupus erythematosus （SLE）. Methods Forty-four SLE patients and 136 other rheumatic diseases patients were studied.The later group included Sjogren＇s syndrome, undifferentiated connective tissue disease, ankylosing spondylitis, and rheumatoid arthritis. Immunoblotting was used to measure the anti-StuD1 antibody, ANuA and anti-SSA60 000 antibody. ANA and anti-dsDNA were detected by indirect immunofluorescence, immunodotting was used to measure the anti-Sm antibody. Results The seropositive rate of anti- StuD1 was 47.7% in SLE patients. It was much higher than that of anti-Sin （P〈0.05）. The specificity of anti- StuD1 was 97.1%. Conclusion The specificity of anti-StuD1 antibody is very high. Anti-StuD1 antibody＇s sensitivity was higher than that of anti-Sin antibody＇s. Combination of the five antibodies can significantly improve the sensitivity of these autoantibodies in the diagnosis of SLE.
出处
《中华风湿病学杂志》
CAS
CSCD
2007年第7期397-401,共5页
Chinese Journal of Rheumatology
