摘要
用液相色谱-电喷雾离子阱串联质谱(LC-ESIIT-MSn)联用法研究阿托品在大鼠肠内菌中的代谢,以阿托品优化色谱及质谱条件,总结其色谱及质谱行为规律。将阿托品与大鼠肠内菌体外厌氧温孵培养,与空白样品及阿托品标准品相比较,根据被测物的多级质谱数据,鉴定代谢物并阐述其结构。在温孵液中发现了阿托品的脱水及水解代谢产物,即脱水阿托品、托品和托品酸。该方法灵敏、快速、简单,适合于药物代谢分析。
Atropine and its metabolites in rat intestinal flora were identified by liquid chromatography and electrospray ionization tandem mass spectrometry （LC-ESI-MSn）. The conditions of chromatographic and mass spectrometric were optimized using atropine standard. The chromatographic and mass spectrometric behavior patterns of atropine were summarized. Atropine was incubated with rat intestinal flora in vitro under lack of oxygen. Identification and structural elucidation of the metabolites were performed by comparing the full scan MS^n spectra of analyzes with those of the parent drug and blank sample. The results reveal that the parent drug and its dehydrolyzed and hydrolyzed metabolites are found in the incubation solution. This method is sensitive, rapid, simple and suitable for analyzing metabolites of drugs.
出处
《质谱学报》
EI
CAS
CSCD
2007年第3期169-173,共5页
Journal of Chinese Mass Spectrometry Society
基金
湖北省中药生物技术重点实验室开放基金(2004CTM001)
湖北大学人才基金项目(020-091130)资助
关键词
液相色谱-串联质谱法
阿托品
代谢物
liquid chromatography-tandem mass spectrometry
atropine
metabolite
