摘要
猪链球菌2型（SS2）是一种重要的人兽共患病病原菌，溶菌酶释放蛋白（MRP）和胞外因子（EF）是其重要的2种毒力因子。参照GenBank上已发表的MRP和EF基因序列，设计引物，对4株国内SS2分离株进行MRP和EF全基因序列测定并进行分析。结果表明：4株SS2国内分离株MRP基因的完整开放阅读框（ORF）都为3771bp、编码1256个氨基酸，EF基因的完整ORF都为2532bp，编码843个氨基酸；与国外参考株D282之间的MRP核苷酸和蛋白质的同源性都为99．9％。EF核苷酸的同源性为99．9％、蛋白质的同源性为99．5％～100．0％。
Streptococcus suis type 2 （SS2） is an important zoonosis agent, of which muramidase-released protein and extracellular protein factor are two important virulent factors. 5 pairs of specific primers were designed and synthesized according to the sequences of MRP gene and EF gene from GenBank. 4 isolates of SS2 were sequenced and analysed. All whole open reading fragment of MRP gene among 4 isolates of SS2 were 3 771 bp encoding 1 256 amino acids. All whole open reading fragment of EF gene among 4 isolates of SS2 were 2 532 bp encoding 843 amino acids. MRP showed a similary of 99. 9% to those of D282 in both DNA sequence and amino acid sequence. EF exhibited the similaries of 99.9% and 99. 5% - 100.0% to those of D282 in DNA sequence and amino acid sequence, respectively.
出处
《江苏农业学报》
CSCD
北大核心
2007年第3期204-207,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家"973"项目(2006CB50443)
江苏省自然科学基金重点项目(BK20060721)
