摘要
目的探讨血红素氧合酶-1(HO-1)对汞引起肾细胞凋亡的影响及其可能的机制。方法64只雄性Wistar大鼠随机分为对照组、单纯染汞组、HO-1诱导组和HO-1抑制组,每组16只。先分别腹腔注射生理盐水、空白液、血晶素、锌原卟啉Ⅸ(ZnPPⅨ)预处理,8 h后处死各组内半数鼠取肾查HO-1表达水平,余鼠除对照组注射生理盐水外,其余3组均腹腔注射HgCl2(2 mg.kg-1),24 h后检测血肌酐(Cr)、尿素氮(BUN)及肾内总抗氧化能力(TAOC)、丙二醛(MDA)、Bcl-2蛋白表达水平与细胞凋亡指数(AI)。结果与对照组比较,单纯染汞组TAOC降低而MDA、Cr、BUN、Bcl-2表达水平和AI均明显增加(P<0.01),HO-1抑制组除Bcl-2表达受抑外,其余指标的变化与单纯染汞组相似,但更为显著。HO-1诱导组与单纯染汞组及HO-1抑制组比较,TAOC及Bcl-2表达均显著升高,而MDA、Cr、BUN及AI则明显降低(均P<0.01)。结论HO-1通过抗氧化、上调Bcl-2蛋白表达水平而对抗汞引起的肾细胞凋亡作用。
Objective To investigate the influence of heme oxygenase-1 （HO-1） on rat renal cell apoptosis caused by mercury and the possible protective mechanisms. Methods Sixty-four male wistar rats were randomly divided into four groups ： control group, simple mercury group, HO-1 induced group and HO-1 inhibited group. Rats in each group were intraperiteoneal injected with saline, vehicle, hemin（ 30 mg· kg^-1）, ZnPPⅨ （20 mg· kg^-1 ） respectively. Eihgt h later, half rats in each group were killed and their kidneys were harvested for detecting the expression and activity of HO-1 protein,the other rats except control group were intraperitoneally injected with 2 mg· kg - 1 mercuric chloride （ HgCI2 ）. Twenty-four h later, blood creatinine （ Cr）, blood urea nitrogen （BUN）,renal malondiadehyde （MDA）,Bcl-2 protein expression and cell apoptosis index （AI） were measured. Results Simple mercury group had decreased total anti-oxidation capacity （TAOC） and inceased MDA, Cr, BUN, Bcl-2 protein expression and AI than those of control group （P 〈 0.01 ）. Except the inhibited Bcl-2 protein expression, all the parameters in HO-1 inhibited group changed in a similar manner as the simple mercury group but to a greater extant. Compared with simple mercury group and HO-1 inhibited group, the TAOC, Bcl-2 protein expression increased and MDA, Cr, BUN, AI decreased significantly in HO-1 induced group （ P 〈 0.01 ）. Conclusion HO-1 could protect against renal cell apoptosis caused by mercury by means of and anti-oxidation and upregulation of Bcl-2 protein expression.
出处
《现代医学》
2007年第4期287-290,共4页
Modern Medical Journal
