摘要
背景与目的:BP1基因是新近发现的转录因子,属于同源盒基因的DLX家族。近年来的国内外研究显示BP1基因与人乳腺癌的发病密切相关。目前BP1基因与细胞周期控制因子之间的关系未见进一步的研究报道。本研究拟观察人乳腺癌组织中BP1的表达情况以及BP1与Cyclin D1表达的关系。方法:收集86例乳腺癌组织和20例癌旁组织,用RT-PCR的方法检测乳腺癌组织和癌旁组织中BP1 mRNA表达的情况。合成BP1多抗,用Western blot方法验证BP1多抗的效果,并检测86例乳腺癌组织中BP1和Cyclin D1免疫组化的情况及BP1蛋白和Cyclin D1蛋白有无共表达。结果:86例乳腺癌组织中,60例呈BP1 mRNA阳性(69.8%)。20例癌旁组织中BP1 mRNA均为阴性。乳腺癌组织中BP1 mRNA表达明显高于癌旁组织(P=0.000)。在86例乳腺癌组织中,Cyclin D1 mRNA表达率为64.0%(55/86),与BP1 mRNA共阳性的有52例,共阴性的有23例,BP1 mRNA和Cyclin D1 mRNA存在共表达(P=0.277)。另外,在86例乳腺癌组织免疫组化检测中,43例呈BP1和Cyclin D1共阳性表达,31例呈BP1和Cyclin D1共阴性。经配对χ2检验显示,Cyclin D1与BP1也存在共表达(P=0.146)。结论:BP1基因在人乳腺癌组织中有较高的表达率,BP1基因和Cyclin D1基因在人乳腺癌组织中有共表达。BP1基因可能是通过调节细胞周期控制因子Cyclin D1导致乳腺癌发生的。
BACKGROUND ＆ OBJECTIVE： BP1, a novel transcriptional factor, belongs to DLX family of homeobox genes. Recent researches showed that BP1 gene is correlated to genesis of breast cancer, but its correlation to cell cycle control factor has not been reported yet. This study was to observe the expression of BP1 in breast cancer, and to make clear its correlation to Cyclin D1. METHODS.. The expression of BP1 and Cyctin D1 in 86 specimens of human breast cancer and 20 specimens of normal breast tissue （3 cm away from primary tumor） was detected by reverse transcription-polymerase chain reaction （RT-PCR）. BP1 poly antibody was made and was certificated by Western blot. The expression of BP1 and Cyclin D1 in 86 specimens of human breast cancer were detected by immunohistochemistry; their correlation was analyzed. RESULTS： The positive rate of BP1 mRNA was significanlty higher in breast cancer than in normal breast tissues （69.8% vs. 0, P 〈 0.001 ）. The positive rate of Cyclin D1 mRNA was 64.0% in breast cancer. BP1 mRNA and Cyclin D1 mRNA were co-expressed in 52 specimens of breast cancer, and simultaneously negative in 23 specimens （P = 0.227）; BP1 protein and Cyclin D1 protein were co-expressed in 43 specimens, and simultaneously negative in 31 specimens （P = 0.146）. CONCLUSIONS： BP1 gene is highly expressed in breast cancer. There is co-expression of Cyclin D1 and BP1 in breast cancer. BP1 gene may promote the genesis of breast cancer through regulating the expression of Cyclin DI.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2007年第7期709-714,共6页
Chinese Journal of Cancer
基金
陕西省国际合作基金资助项目(No.2004KW-22)~~
