摘要
目的:观察血小板经不同mPEG修饰后功能变化情况,并分析其原因。方法:分别用mPEG-BTC、mPEG-SPA对血小板进行化学修饰;流式法检测修饰前后血小板CD62P荧光强度变化;并对血小板的聚集、黏附及释放功能进行检测。结果:经mPEG-BTC、mPEG-SPA修饰后,血小板CD62P表达率由修饰前的(12.77±2.87)%分别降至0和(4.12±1.28)%;聚集能力由修饰前的(1.43±0.74)%分别升至(3.86±1.21)%和(3.84±1.12)%;黏附能力由修饰前的(30.2±5.59)%分别升至(45.5±2.72)%和(45.2±3.99)%;而血小板Ca2+释放能力修饰前后差异无统计学意义。结论:使用mPEG-SPA对血小板进行化学修饰可影响血小板的功能,是一种较为理想的修饰剂。
Objective：To investigate the metergasis of platelets camouflaged by methoxy polyethylene glycols（mPEG） with different active groups, and to analyze its mechanism, nethods：Platelets were camouflaged by mPEG benzotriazole carbonate（mPEG-BTC） or succinimide of mPEG propionic acid（mPEG-SPA）. The activated molecule CD62P of platelets was detected by flow cytometry and the platelet functions of aggregation, adhesiveness, and release were detected as well. Results：After the platelets were camouflaged by mPEG-BTC or mPEG-SPA, the expression rate of CD62P dropped from pre-modificatoin （ 12. 77 ± 2. 87） % to post-modification 0 and （4.12 ± 1.28 ） %, respectively. But the ability of aggrega- tion was enhanced from pre-modificatoin （ 1.43 ± 0. 74） % to post-modification （ 3.86 ±1.21 ） % and （ 3.84 ±1.12 ） %, and the ability of adhesion was enhanced from pre-modificatoin （30.2 ±5.59 ）% to post-modification （45.5 ± 2.72 ）% and （45.2 ±3.99 ） % respectively. The ability of Ca^2＋ release was not influenced by the modification. Conclusion： The effect on the metergasis of platelets camouflaged by mPEG-SPA was less than that by mPEG-BTC, mPEG-SPA was a suitable modifier to camouflage platelets.
出处
《军事医学科学院院刊》
CSCD
北大核心
2007年第3期235-236,242,共3页
Bulletin of the Academy of Military Medical Sciences
