摘要
目的:观察当归芍药散(DSS)对血脂异常大鼠抗氧化能力及动脉壁血管细胞黏附分子-1(VCAM-1)mRNA表达的影响。方法:采用高脂饲料喂养法复制血脂异常大鼠模型,同时给予治疗药物DSS(汤剂)高、低剂量及阳性对照药血脂康,连续8周,检测各组大鼠血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及氧化型低密度脂蛋白胆固醇(ox-LDL)、丙二醛(MDA)、超氢化物歧化酶(SOD)水平,并用反转录聚合酶链式反应(RT-PCR)法测定大鼠主动脉VCAM-1mRNA的表达。结果:与模型组相比,DSS高、低剂量组和血脂康组均能明显降低大鼠血清TC、TG、LDL-C及ox-LDL、MDA水平(P<0.01),均能明显升高HDL-C、SOD水平(P<0.01)。RT-PCR法显示DSS高、低剂量组和血脂康组均能显著降低大鼠主动脉组织中VCAM-1mRNA的表达(P<0.01),其中DSS高剂量组降低趋势优于低剂量组和血脂康组。结论:DSS高、低剂量组均可较好地调节血脂异常大鼠脂质代谢,清除自由基、抗脂质过氧化损伤并下调动脉壁VCAM-1基因的表达,提示其具有保护血管内皮细胞功能的作用,可进一步阻抑动脉粥样硬化的发生发展。
Objective： To observe the effect of Danggui Shaoyao San （DSS） on lipid＇s peroxidation and the expression of VCAM-1mRNA in dyslipidemia rats. Methods： Hyperlipemia of rat model was duplicated by feeding high lipid fodder,at the same time, the rats were treated with DSS and positive control medicine. Eight weeks later, blood lipids level as well as the content of ox-LDL, MDA and SOD were detected by RIA kit. The expression of the VCAM- 1 mRNA was detected by RT-PCR. Results： The TC ,TG, LDL-C ,ox-LDL and MDA in DSS group and the positive control group were decreased while the HDL-C and SOD were increased, compared with the model group（ P 〈 0.01 or P 〈 0.05）. The result of RT-PCR showed that DSS and positive control group could inhibit the VCAM-1 mRNA＇ expression in the rat aorta. Conclusion：DSS can inhibit lipid＇s peroxidation damage and decrease the expression of VCAM-lmRNA in rats. The results indicated that DSS could protect the VEC＇s function.
出处
《中国实验方剂学杂志》
CAS
2007年第2期25-28,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省科技厅攻关计划项目(03276196D-4)
