摘要
白术(Atractylodes macrocephala Koidz)水溶性粗多糖,经乙醇分级及Sepha-dex G-75层析柱制备得到组分AMP.经Sephadex G-75、DEAE-阴离子交换纤维素层析及HPLC验证,AMP相对分子质量及极性为均一组分,HPLC测得相对分子质量为8 374.气相色谱(GC)分析表明其单糖组成为Glc,Gal,Rha和Man,物质的量比为7.36∶1.00∶3.05∶1.52.多糖AMP经部分酸水解、高碘酸氧化、Smith降解、甲基化分析,其结构主体部分由Glc,Rha和Man组成,平均每6个残基上有1个分支,分支点为Glc的4-O处和Man的6-O处,末端残基为Glc.
The water soluble crude polysaccharide was seperated from Atractylodes macrocephala Koidz by water. After ethanol precipitation, Sephadex G-75 gel filitration, the fraction of AMP was obtained. By analyzing of Gel filtration on Sephadex G-75, ion-exchange chromatography on DEAE column and HPLC, the homogeneous of AMP was determined. The molecular weight of the AMP is 8 374 measured by HPLC. GC analysis indicated that AMP was composed of Glc,Gal, Rha and Man, the molar ratio was 7. 36：1.00 ： 3. 05 ： 1.52. By analyzing the products of partial hydrlsys,Smith degradation, methylation and GC-MS analysis, the basic structure of AMP was determined as Glc,Rha and Man. On average, there was 1 branch among 6 main chain residues, which was substituted at 4-0 of Glc and 6-O of Man. The nonreduced end was Glc.
出处
《分子科学学报》
CAS
CSCD
2007年第3期185-188,共4页
Journal of Molecular Science
基金
国家自然科学基金资助项目(30570417)
关键词
白术
多糖
结构
Atractylodes macrocephala Koidz
polysaccharide
structure
