摘要
The concentrations of bovine serum albumin,gliadin,bovine hemoglobim,mucin,lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm(A230 assay).For all the sample solutions of pure proteins,the linear range of A230assay was 25～250μg·mL-1,and salt had little influence on this method.For the solutions containing two kinds of proteins,the mass ratio of the proteins affected the result of determination.For the solutions of proteins mixture,the average recovery rate(n=6)of A230assay was 101.6%(99.04%～109.1%)with RSD=3.82%.As a result,a new effective method to determine protein concentration was suggested.
The concentrations of bovine serum albumin, gliadin, bovine hemoglobim, mucin, lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm（ A230 assay）. For all the sample solutions of pure proteins,the linear range of A230assay was 25 -250μg · mL^-1 ,and salt had little influence on this method. For the solutions containing two kinds of proteins, the mass ratio of the proteins affected the result of determination. For the solutions of proteins mixture, the average recovery rate（n =6）of A230 assay was 101.6% （99. 04% ～ 109. 1% ）with RSD =3. 82%. As a result,a new effective method to determine protein concentration was suggested.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2007年第5期562-566,共5页
Chemical Research and Application
基金
"973"重大基础前期研究专项(2004CCA06100)资助
国家自然科学基金项目(20476062)项目资助
国家自然科学基金重点项目(20536030)资助
