摘要
目的初步了解肠道病毒（EV）在小儿急性呼吸道感染（ARI）中的流行概况。方法2003-09—2005-04,于首都儿科研究所就诊的815例ARI患儿,取其鼻咽深部分泌物,用病毒分离及逆转录聚合酶链反应（RT-PCR）法检测EV,用病毒分离和间接免疫荧光法检测呼吸道合胞病毒（RSV）等7种呼吸道病毒。取144例患儿双份血清,用酶联免疫吸附试验检测EV抗体。结果病毒总检出率50.9%,RSV阳性率最高（24.9%）,其次为EV（16.9%）。EV在不同月份阳性率为0~34.2%,冬季阳性率最低。RT-PCR、双份血抗体检测和病毒分离3种检测EV方法的阳性率分别为16.2%、7.6%和1.0%。332例急性喘息患儿中,EV阳性率20.8%,仅次于RSV（42.5%）;3岁以上喘息患儿中,EV阳性率最高（33.9%）,而3岁以下患儿中,RSV阳性率最高（48.7%）。结论在住院ARI患儿中,EV是仅次于RSV的常见病原,也是引起小儿急性喘息性疾病的主要病原之一。EV在年长儿中阳性率相对较高,冬季阳性率较低。RT-PCR法检测EV,快速、敏感、特异,可用于小儿EV感染的诊断。
Objective To investigate the prevalence of enterovirus （ EV ） in acute respiratory infections （ ARI ） in children. Methods Nasopharyngeal secretion samples from 815 inpatient children with ARI were obtained and were detected EV by both viral culture and reverse transcription polymerase chain reaction （ RT-PCR ）. The samples were also used tO detect RSV,influenza virus type A and B, parainfluenza virus type 1,2 and 3, and adenovirus by both viral culture and in- direct immunofluorescence assay. Results The viral positive rate of all cases was 50.9% ,with RSV （ 24.9% ） being the most prevalent,followed by EV （ 16.9% ）. The prevalence of EV were 0 - 34. 2% in different months,which was the lowest in winter. The positive rate of RT-PCR, paired serum antibody detection and virus culture in detecting EV infection was 16.2% ,7.6% and 1.0% ,respectively. Conclusion Among inpatient children with ARI,EV is the second most common viral pathogen detected following RSV, and is also one of the important viral pathogens causing acute expiratory wheezing illness in children. The prevalence of EV is the lowest in winter in Beijing. RT-PCR is a rapid, sensitive and specific method. It can be used as a useful tool in detecting EV-associated ARI in children.
出处
《中国实用儿科杂志》
CSCD
北大核心
2007年第4期292-294,共3页
Chinese Journal of Practical Pediatrics
关键词
儿童
急性呼吸道感染
肠道病毒
逆转录聚合酶链反应
Child
Acute respiratory infection
Enterovirus
Reverse transcription polymerase chain reaction
