摘要
目的探讨肿瘤坏死因子(tumor necrosis factor,TNF)A-308位点基因多态性与侵袭性牙周炎的关系。方法选择64例侵袭性牙周炎(aggressive periodontitis,AgP)患者及78名健康对照者,提取其外周静脉血基因组 DNA,采用多聚酶链反应-限制性内切酶片段长度多态性的方法检测TNF A-308位点的基因多态性。结果 TNF A-308位点的 GA 基因型频率在两组间差异无统计学意义;但是在按性别和吸烟分层的条件下,携带基因型 GA 和等位基因 A 使男性不吸烟者患病的风险明显增加(OR 值分别为22.2和16.1)。结论提示 TNF A-308基因型 GA 和等位基因 A 可能与中国人群中男性个体的 AgP 易感性有关。
Objective To investigate the relationship between tumor necrosis factor （TNF） A-308 gene polymorphism and aggressive periodontitis （AgP）. Methods Anti-coagulated peripheral blood samples were obtained from 64 AgP patients, and 78 healthy controls （H）. Genomic DNA was extracted from each sample. The TNF A-308 gene polymorphism was detected by standard polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） assay. Data were analyzed by SAS statistical software. Results There was no significant difference in distribution of TNF A-308 genotype between AgP patients and healthy controls. But the ORs for genotype GA and allele A were 22. 2 and 16. 1 respectively in non-smoking male AgP patients. Conclusions The TNF A-308 genotype GA and allele A may be associated with AgP susceptibility in Chinese males.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2007年第5期268-271,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金(30271411
30471882)
卫生部部属(管)医疗机构2004-2006年临床学科重点项目
关键词
肿瘤坏死因子
牙周炎
多态性
限制性片段长度
疾病易感性
Tumor necrosis factor
Periodontitis
Polymorphism, restriction fragment length
Disease susceptibility
