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实时荧光定量PCR技术运用于肝癌患者基因检测 被引量：6

Application of Real-time Quantitative PCR in MDS Patients Detecting
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摘要 目的探讨实时荧光定量PCR SYBR Green I探针技术在研究肝癌患者中基因表达情况的可行性。方法运用实时荧光定量PCR SYBR Green I技术,检测肝癌患者癌组织及癌旁组织RAP1GAP基因表达情况。结果实验组和对照组有明显的统计学差异。结论Real-time PCR技术检测目的基因的表达是非常方便快捷准确的方法。 Objective To explore the feasibility of real-time quantitative PCR （QRT-PCR） in MDS patients detecting.Mehtods Real-time quantitative polymerase chain reaction （RQ-PCR） assay was established and performed to measure the transcript level of RAP1GAP in tissues of live cancer and normal.Results RAP1GAP transcripts were significantly different in tissues of liver cancer and the normal.Conclusion The real-time RT-PCR method is currently the most sensitive and precise method for quantification of RNA in the cell.
作者 张韵红
机构地区 苏州高新区疾病预防中心
出处 《中国血液流变学杂志》 CAS 2007年第1期135-136,共2页 Chinese Journal of Hemorheology
关键词 实时荧光定量PCR 肝癌 RAP1GAP基因 Real-time PCR live cancer RAPIGAP
分类号 R730.43 [医药卫生—肿瘤] R735.7 [医药卫生—肿瘤]
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参考文献9

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二级参考文献50

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共引文献28

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引证文献6

二级引证文献78

中国血液流变学杂志
2007年 第1期

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